摘要
A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.
A highly sensitive and simple Rayleigh light scattering （RLS） method for the determination of ciprofloxacin hydrochloride has been developed. Ciprofloxacin hydrochloride （CPFH） reacted with tetraphenylboron sodium（TPB） to form an ion-association complex in pH 5.31 aqueous solution, which resulted in a significant enhancement of the RLS intensity, which was linear with the concentration of CPFH in the range of 12.7-419 ng·mL^-1. The limit of detection for CPFH was 6.99 ng·mL^-1. The method is simple, highly sensitive and selective within a wide linear range. Applying this method for the determination of ciprofloxacin hydrochloride in pharmaceutical preparations and biological fluids obtained satisfactory results.
基金
Supported by the National Natural Science Foundation of China(20675059)
Research Foundation of Leshan Teachers’ College, China (Z0625)
