庆大霉素对截瘫大鼠肠道细菌移位影响的实验研究

Effect of gentamicin on bacterial translocation after paraplegia caused by spinal cord injury in rats

目的探讨庆大霉素灌胃和肌注两种不同给药方式对脊髓损伤并发截瘫大鼠肠道细菌移位的影响。方法建立大鼠脊髓损伤性截瘫模型,以庆大霉素灌胃治疗和肌注治疗两组大鼠为实验组,以脊髓损伤生理盐水灌胃和肌注两组大鼠为相应对照组。采集动物下腔静脉血进行内毒素定量检测和细菌培养,采集肝、脾、肠系膜淋巴结、肠内容物作细菌培养并进行菌种鉴定。取实验组和对照组各动物的肝、脾、肠系膜淋巴结、空肠、回肠进行病理切片及染色检查。结果庆大霉素灌胃组内毒素低于其他三组,血细菌培养仅生理盐水灌胃组阳性率高,脾细菌培养仅生理盐水肌注组阳性率高;肠系膜淋巴结细菌培养、庆大霉素灌胃组阳性率低,而生理盐水灌胃组阳性率高。结论本研究提示在预防及治疗脊髓损伤性截瘫后并发细菌?内毒素时应以经胃肠道途径给药为主。

Objective The present study was investigated the effects of gentamicin on bacterial translocation in spinal cord injury in rats. Methods The paraplegia rats induced by injuring spinal cord were divided into two groups, including experiment group and control group. The experimental group was treated with gentamiein and the control groups with physiological saline as control. Blood, liver, spleen, mesenteric lymph node and intestinal contents were collected for bacterial culture and endotoxin detection. The samples of liver, spleen, mesenteric lymph node, jejunum and ileum were collected for pathology study. Results The endotoxin levels and the positive rates of bacterial culture in lymph nodes and blood in the experimental groups demonstrated statistical difference compared with that of control （P〈0.05）, Con-clusion Antibiotics should be administered to paraplegia patients with spinal cord injury by way of gastrointestinal tract.