超高效液相色谱-四极杆-飞行时间质谱对混合光合作用糖脂的分析

Ultra Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometric Analysis of Photosynthetic Glycolipids Mixture

采用电喷雾电离源(ESI),分别在正离子模式下对混合单半乳糖甘油二酯、双半乳糖甘油二酯和在负离子模式下对混合硫代异鼠李糖甘油二酯进行超高效液相色谱-四极杆-飞行时间质谱的定性定量研究。结果表明,采用梯度洗脱,各组分在BEHC18柱上可得到良好分离,根据各特征碎片离子可以确定糖脂的种类和酰基组成,根据特征碎片离子信号强度之间的关系,可进一步确定各酰基的位置;采用等梯度洗脱可确定各组分占总组分的百分组成,从而测定出各组分在混合脂中的绝对含量。

Qualitative and quantitative analysis of photosynthetic glycolipids mixture was studied by means of the ultra performance liquid chromatography-quadrupole-time of flight mass spectrometry （UPLC-Q-TOF-MS） using electron spraying ionization. The results showed that the ionization mode was depending on the structural types, namely, monoglycosyldiglycerides（MGDG） and diglycosyldiglycerides （DGDG） in positive ion mode, whereas sulfoquinovosyl diacylglycerol （SQDG） in negative ion mode. Each glycolipid component in the mixture can be separated effectively using gradient elution on BEH C18 column. The characteristic fragment ions were shown in the mass spectra indicating the polar group and acyl chains, the relative intensity of the two acyl chains were clearly correlated with their regiostereochemsitry. The quantitation of glycolipid has been carried out by accounting their composition in isocratic elution.