穴位埋药线和电针干预对脑缺血再灌注大鼠海马热休克蛋白70表达的影响

Effects of Embedding Medicine in Acupoints and Electro-acupuncture on Expression of HSP70 in Hippocampus of Rat with Focal Cerebral Ischemia Reperfusion

目的观察局灶性脑缺血再灌注大鼠海马热休克蛋白70(HSP70)表达及川芎嗪缓释剂穴位埋药线和电针干预对其的影响。方法将SD大鼠随机分为正常组、假手术组、模型组、电针组、穴位埋药线组。采用改良线栓法制备局灶性脑缺血再灌注模型,予电针及以胶原川芎嗪制备的缓释剂(药线)在大椎、双侧内关穴位埋药线。应用免疫组化方法检测不同时相(24、72、120h)各组大鼠海马HSP70的表达。结果正常组及假手术组大鼠海马仅见少量HSP70阳性细胞,治疗各组大脑海马HSP70表达均增加,24h时HSP70阳性细胞数达到高峰,以后逐渐下降,与模型组比较差异有统计学意义(P<0.01)。其中穴位埋药线组大脑海马HSP70表达最为明显,与电针组比较差异有统计学意义(P<0.05)。结论川芎嗪穴位埋药线可诱导脑缺血再灌注后HSP70表达,进而抑制细胞凋亡,作用优于电针组。

Objective To observe the effects of embedding implantation of collagen and Tetramethylpyrazine in acupoints and electro-acupuncture on the expression of HSP70 in hippocampus of rat with focal cerebral ischemia reperfusion. Methods SD rats were randomly divided into normal group, sham operation group, model group, electro-acupuncture group, embedding medicine in acupoints group. Model of local ischemia was made by middle cerebral artery occlusion, preparation of implantation collagen and electro- acupuncture and Tetramethylpyrazine was embedded in the acupoints of Dazhui and Neiguan two-sided. The expression of HSP-70 protein in hippocampus of different time point （24, 72, 120 h） was assayed by immunohistochemistry. Results There were only few positive cells of HSP-70 in normal group and sham operation group. The expression of HSP-70 in each therapy group were all heightened, and achieved the peak 24 h later and then declined in brain tissue after focal cerebral ischemiareperfusion, the differences were relatively prominent compared with the same phase model group （P〈0.01）. The expression of HSP-70 increased in embedding medicine group significantly compared with the electro-acupuncture group （P 〈0.05）. Conclusion Embedding implantation of collagen and Tetramethylpyrazine in acupoints can increase the expression of HSP-70 in hippocampus of rats with cerebral ischemia reperfusion, which may protect neuronal cells after cerebral ischemia reperfusion in rats.