大孔吸附树脂分离纯化长春花中长春碱和长春新碱

Isolation and Purification of Vinblastine and Vincristine from Gatharanthus Roseus by Macroporous Adsorption Resin

目的优选分离纯化长春花中长春碱和长春新碱的最佳工艺参数。方法采用不同型号大孔吸附树脂对长春花中长春碱和长春新碱分离纯化,用不同浓度乙醇水溶液以不同速度洗脱,配合硅胶柱层析,以HPLC跟踪检测。结果AB-8型树脂分离效果佳,90%乙醇水溶液以1.5BV/h的速度洗脱,总比吸附量达到74.5mg/g,经硅胶柱层析后长春新碱纯度达97.26%,长春碱纯度达94.18%。结论该工艺简便合理,重复性良好,能得到高纯度的长春碱和长春新碱。

Objective To optimize the technological parameters of separation and purification of vinblastine and vincristine from Gatharanthus Roseus. Methods Different types of macroporous adsorption resin were used to separate and purify vinblastine and vincristine from Gatharanthus Roseus, eluting with different concentration of alcohol aqueous and velocities, combined with silica gel column chromatography, the process was monitored by HPLC. Results AB-8 type resin showed better comprehensive adsorption property, 90% alcohol aqueous and 1.5 BV/h velocities were used to elute. Ratio adsorption quantity was achieved to 74.5 mg/g. Through silica gel column chromatography, the purity of vincristine was achieved to 97.26% and the purity of vinblastine was achieved to 94.18%. Conclusions The process is simple and reasonable with good reproducibility. It is effective to enrich highly purified vinblastine and vincristine.