水环境中病原微生物PCR法检测原理及应用进展

The principle and application progress of PCR technique about pathogenic microbes in water environment

水中的病原微生物威胁着人类的健康安全,需要准确检测。分子生物学方法中PCR法具有不可比拟的优点,是最普遍的微生物分子生物学检验方法。目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术,各种技术有不同的原理及优缺点。

The pathogenic microbes in water have threaten people＇s health and needed to be exactly detected. PCR technique has particular advantage in molecular biological methods and is the most widespread method. At present, the common methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.