疏肝理气通络法对小鼠产后缺乳发病机制的调控

The regulation of soothing the liver qi and dredging collaterals to pathogenesis of mice postpartum hypogalactia

目的:探讨疏肝理气通络法对肝郁气滞产后缺乳动物模型发病机制的影响以及疗效观察。方法:取普通健康昆明小鼠45窝,其中母鼠45只,并随机分为中药组、对照组和空白组,用催乳素中枢抑制剂溴隐亭制作肝郁气滞缺乳模型。从产后第3天开始,将溶于生理盐水的溴隐亭配制成0.02mg/ml浓度的溶液,按每只0.5mg/(kg·d)的剂量给中药组和对照组母鼠灌胃造模,连续8d。在这期间每天8∶00～20∶00单独给予中药组和对照组母鼠声光刺激,每2小时1次,使其因受到惊吓而影响泌乳。其中单独给予中药组母鼠每天插胃管饲服"肝舒涌乳汤"(自拟方)1ml,每天2次。仔鼠至产后第2、5、10天分别测量小鼠体重,每窝取平均数。第11天处死小鼠,采血检测催乳素(PRL)、雌激素(E2)及孕激素(P)水平;取母鼠乳腺做组织形态学观察。结果:对照组催乳素水平明显低于中药组和空白组(P<0.01),而雌激素及孕激素水平明显高于中药组和空白组(P<0.01);组织形态学显示:对照组乳腺小叶腺泡减少,结缔组织和脂肪组织增多;中药组与空白组乳腺形态相仿,小叶腺泡充满乳汁而显著扩大,小叶间质的结缔组织明显减少。结论:疏肝理气通络复方中药能改善溴隐亭导致的缺乳母鼠的泌乳功能。

Objective：To discuss the influence and observe the effects of soothing the liver qi and dredging collaterals to pathogenesis of postpartum hypogalactous animal model with liver depression and qi stagnation.Methods：45 broods of ordinary healthy Kunming mice, including 45 maternal mice, were selected and randomly divided into three groups：treatment group with Chinese medicine, control group and blank group, and established hypogalactous model of liver depression and qi stagnation with Bromocriptine, the prolactin central inhibitor.Bromocriptine was dissolved with normal saline and prepared it according to the concentration of 0.02 mg/ml, from the third day postpartum maternal mice in control and blank groups began to be administrated with Bromocriptine by gavage dosage of 0.5 mg/（kg·d） each one, this administration continued for eight days.During this period stimulated them once every 2 hours from 8∶00 to 20∶00 with sound and light to influence their lactation for affright.Administrated 1 ml twice each day to mouse in treatment group with Soothing Liver and Promoting Lactation Decoction（self-prescription） by gastrogavage, then the weights of newborn mouse in 2ed , 5th and 10th day postpartum were measured, and the average weight of every brood was calculated.Maternal mice were sacrificed and drawn blood in 11th day and the levels of prolactin（PRL）, estrogen（E2） and progesterone（P） in blood were detected.Histomorphology of mammary glands of maternal mice were also observed.Results：The level of PRL in control group was distinctly lower than that of treatment group and blank group（P〈0.01）, meanwhile, the levels of E2 and P in control group were higher than treatment group and blank group（P〈0.01）.The histomorphology of mammary glands indicated that the acini of mammary lobule decreased but connective tissue and fat tissue increased in control group, the morphology of treatment group and blank group were similar that the acini of mammary lobule enlarged, filling with latex, connective tissue of lobular mesenchymal decreased obviously.Conclusion：Compound Chinese medicine soothing liver, regulating qi and dredging collaterals can improve the lactation function of hypogalactous maternal mice induced by Bromocriptine.