摘要
目的探讨血管内皮生长因子(VEGF)在SD慢性神经痛大鼠脊髓对伤害性刺激反应的作用。方法应用热板(52℃)和机械压力实验法,以大鼠后爪缩爪反应潜伏期(HWL)为痛阈指标,观察鞘内微量注射VEGF或SU1498后的HWLs的变化。结果鞘内注射0.2、0.4 nmoL VEGF显著降低大鼠对热板和机械压力刺激的双侧HWLs,且呈量效关系;鞘内微量注射SU1498可逆转VEGF的易化作用。结论鞘内微量注射VEGF使大鼠对热板和机械压力刺激发生痛敏,其作用可能通过VEGFR2/Flk-1受体。
Objective To investigate the effects of microinjection of vascular endothelial growth factor （VEGF） into theeae on the nociceptive responses in mononuropathic rats. Methods The latency of hindpaw withdrawal（HWL） from the hot-plate（52℃） and the mechanical test in SD rats were used as an index to measure the pain threshold, the effect of microinjection of VEGF, SU1498 into theeae on HWLs was observed. Results Intrathecal administration of 0.2 and 0.4 nmoL VEGF significantly induced bilateral decrease in HWLs in dosedependent manner. Administration of 0.4 nmoL SU1498 significantly may reverse VEGF-induced decrease in bilateral HWLs. Conclusion The results suggest that VEGF plays a facilitating role on the HWLs to nociceptive stimulation and that the VEGFR2/Flk-1 receptor in spinal cord participate in an interaction in the transmission of nociceptive information of mononeuropathic rats.
基金
山东省科技攻关计划资助课题(2006GG3202044)
山东省自然科学基金资助(Y2002C05)
