摘要
在pH=6.2的Na2HPO4-NaH2PO4缓冲溶液中,羊抗人纤维蛋白原血清与纤维蛋白原(Fg)发生特异性结合,产生抗体抗原免疫复合物微粒,导致体系在340 nm处的共振散射峰增强。当纤维蛋白原质量浓度在1.33×10^-7~5.33×10^-6g/mL范围内,随着纤维蛋白原浓度c的增加340 nm处的共振散射强度线性增强,回归方程为ΔI340 nm=12.17c+3.35,相关系数为0.998 6,其检出限(3σ)为2.8×10^-8g/mL Fg。考察了18种共存物质的干扰。结果表明,方法具有较高的选择性。方法用于定量分析人血浆中的纤维蛋白原,相对标准偏差在2.2%~2.3%之间,测定结果与人体正常血浆中纤维蛋白原含量基本一致。
In a pH 6.2 Na2HPO4-NaH2PO4 buffer solution,the immune reaction of goat anti-human fibrinogen serum and human fibrinogen(Fg) forms immune complex particles that exhibit a stronger resonance scattering peak at 340 nm.The resonance scattering intensity at 340 nm was enhanced with the increase of concentration of Fg.There is a linear relation in resonance scattering intensity ΔI340 nm to the Fg concentration c in a range of 1.33×10^-7~5.33×10^-6 g/mL.The regressed linear equation is ΔI340 nm=12.17c+3.35,the relation coefficient is 0.998 6.The detection limit(3σ) is achieved at 2.8×10^-8 g/mL Fg.Influence of 18 substances on the determination was examined.Results indicate that this assay has a good selectivity.The proposed method was applied to the determination of Fg in plasma,with a relative standard deviation of 2.2%~2.3%.The result is in agreement with the Fg content in normal plasma.
出处
《应用化学》
CAS
CSCD
北大核心
2009年第4期451-455,共5页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金(20365001,20667001)
广西新世纪十百千人才计划(2004206)资助项目
关键词
纤维蛋白原
免疫反应
共振散射光谱法
fibrinogen,immunoreaction,resonance scattering spectral assay