山楂果和桑枝提取物中总黄酮含量的Fe(phen)_3^(2+)探针共振瑞利散射法测定

Resonance Rayleigh Scattering Method with Fe(phen)_3^(2+) as a Probe for Determination of the Content of Total Flavones in Hawthorn Fruit and Ramulus Mori

在pH值约为8.0的BR缓冲介质中,桑色素、槲皮素和芦丁等黄酮类物质能与Fe(phen)32+反应形成离子缔合物,此时将引起共振瑞利散射(RRS)显著增强,最大RRS波长均位于310 nm。在一定范围内散射强度(ΔI)与黄酮类物质的浓度成正比,据此可以建立用Fe(phen)32+测定某些黄酮类物质的新RRS法。对桑色素、槲皮素和芦丁的检出限(3σ/K)分别为1.25×10-7mol/L(37.74μg/L),1.80×10-7mol/L(54.42μg/L),2.72×10-7mol/L(167.30μg/L)。研究了反应的适宜条件和影响因素,考察了共存物质的影响。结果表明,方法有较好的选择性。分别以芦丁和桑色素为对照品测定了中药材山楂果和桑枝中的总黄酮含量,对反应机理进行了初步探讨。

In a pH=8.0 BR buffer solution,some flavones such as morin,quercetin,and rutin,can react with Fe（phen）2＋3 to form ion association complexes.As a result,the Resonance Rayleigh scattering（RRS） is enhanced greatly.The maximum RRS wavelengths of the three ion association complexes are all located at 310 nm.In a certain concentrations range,there is a linear relationship between the RRS intensity and the flavones concentration.So,a new RRS method for the determination of some flavones with Fe（phen）2＋3 as a probe was established.The method had a high sensitivity and selectivity,with the detection limit（3σ/K） of 1.25×10^-7 mol/L（37.74 μg/L） for morin,1.80×10^-7 mol/L（54.42 μg/L） for quercetin and 2.72×10^-7 mol/L（167.30 μg/L） for rutin,respectively.The optimum reaction conditions and the effects of foreign substances were investigated.The contents of total flavones were determined in Hawthorn Fruit and Ramulus Mori with rutin and morin as the reference compounds respectively.In addition,the reaction mechanism was discussed.