宽叶菘蓝体细胞无性系根腐病抗性的RAPD初步分析

Initial RAPD Analysis of Isatis indigotica Somatic Clones' Resistance to Root Rot Disease

目的:初步鉴定宽叶菘蓝体细胞无性系植株的抗病突变体。方法:在一定浓度根腐病菌粗毒素的胁迫下诱导抗性植株。选取同一个无性系植株及其亲本进行随机扩增多态性DNA(RAPD)分析。结果:初步获得有抗性的植株,12个引物获得较为清晰的扩增谱带。其中大部分引物扩增产物无差异,但也有少部分引物扩增产物有差异。无性系植株的遗传背景一致性很好,但也发生了DNA水平的变异。结论:本项结果可为进一步鉴定宽叶菘蓝抗性突变体和分离抗病基因提供参考。

Objective： To make initial identification on Isatis Indigotica Somatic Clones＇ resistant mutant. Methods： The resistant plants were selected under the treatment of root-rot disease crude toxin. The DNA of the same clones and their parents were analysed by RAPD technology. Results： We got initial resistance of the plants and selected 12 primers of which most were identical but the less were different. The genetic background of the somatic clones was identical but the individuals were mutated on the DNA level. Conclusion： It provides experimental foundation for further study on separating resistant mutant and resistant gene.