姜黄素经红曲霉菌发酵后对高脂血症小鼠脂代谢的影响

Study of the Property of Lipids Reducing of Curcumin on Hyperlipidemia Mice after Fermented by Monascus purureus

目的:利用红曲霉菌对姜黄素进行发酵,研究发酵后姜黄素对高脂血症小鼠脂代谢的影响。方法:选择本实验室保存的不产洛伐他汀的红曲霉菌,制成适量的孢子悬液,加入到装有1%姜黄素的发酵培养基中,恒温培养进行姜黄素的发酵。设只含1%姜黄素而不接种菌株的底物对照,考察转化液中是否存在非微生物引起的药物分解产物;设不含姜黄素的菌株对照,排除菌株的发酵产物和培养基残留成分的干扰。昆明种小鼠喂饲高脂饲料15d形成高脂血症模型,以高、中、低剂量(质量浓度分别为500、200、100mg/kg)的发酵姜黄素灌胃;设底物对照和菌体对照治疗组(500mg/kg);模型组、正常组等容积生理盐水灌胃。治疗10d后禁食12h,摘眼球取血,酶法测定TC、TG、HDL-C;取肝脏匀浆后,提取上清液,测定TC、TG、HDL-C。结果:发酵后姜黄素高、中、低剂量治疗均能显著减低高脂血症小鼠血清TC、TG,血清TC降低幅度分别为38.7%、34.5%、32.7%,TG降低幅度分别为38.3%、28.6%、30.1%,而底物对照组和菌体对照组的治疗效果不明显。发酵后姜黄素亦能降低高脂血症小鼠肝脏TC、TG,且同等质量浓度的底物姜黄素疗效不明显。结论:姜黄素发酵后能显著提高高脂血症小鼠的血脂调节能力。

Objective ：To study the tipids reducing property of curcumin on Hyperlipidemia mice after fermented by Monascus pururetts. Methods：The stain Monascus purureus was used for microbial transformation, and both substrate control and strain control were set. The mice were reared with high lipid and cholesterol feed for 15d to establish the Hyperlipidemia models. The models were treated with fermented curcumin in 500 mg/kg,200 mg/kg, 100 mg/kg, substrate control and strain control in 500 mg/kg. Positive and Normal