丙泊酚对实验性脑出血大鼠的脑保护作用(英文)

Protective effect of propofol against intracerebral hemorrhage injury in rats

为研究丙泊酚对实验性脑出血大鼠的脑保护作用,本研究采用左侧尾状核注射胶原酶0.5U(1U·μL-1)诱导大鼠脑出血模型,并在胶原酶注射前10min腹腔注射给予丙泊酚。观察丙泊酚对大鼠脑出血后神经行为学评分,脑含水量,脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及caspase-3表达的影响。结果显示,与脑出血组大鼠相比,丙泊酚(30和100mg·kg-1)可不同程度地减小脑出血大鼠神经行为学评分(P<0.05,P<0.01),降低脑组织含水量(P<0.05),提高脑组织SOD活性(P<0.05),降低脑组织MDA含量(P<0.01)。丙泊酚(15,30及100mg·kg-1)可呈剂量依赖性地抑制caspase-3的表达(r=0.877)。提示丙泊酚对大鼠脑出血损伤具有一定的保护作用,其机制可能与丙泊酚的抗氧化特性有关。

The neuroprotective effect of propofol against intracerebral hemorrhage （ICH） in rats was investigated. ICH was induced in rats by infusion of collagenase （Type Ⅶ ） 0.5 U （1 U·μL^-1） into the left caudate nucleus. Three doses of propofol were given intraperitoneally （ip） 10 min before collagenase infusion. Effects of propofol on neurological behavioral scores, brain water content （BWC）, activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） in brain tissue, expression level of caspase-3 were studied. In propofol groups （30 and 100 mg·kg^-1）, the neurological behavioral score, BWC and the content of MDA were significantly lower than those in ICH group （P 〈 0.05, P 〈 0.01）, whereas the activity of SOD was higher than that in ICH group （P 〈 0.05）. Meanwhile, propofol （15, 30, and 100 mg·kg^-1） inhibited caspase-3 expression in dose-dependent manner （r = 0.877）. Brain damages caused by ICH in rats can be alleviated by propofol, which mechanism might be attributed to its antioxidant activity.