三氯乙烯对裸鼠皮肤中NO含量的影响及银杏叶提取物和维生素E的作用

Changes of nitric oxide after trichloroethylene irritation in hairless mice skin and protection of ginkgo biloba extract and vitamin E

目的研究三氯乙烯（TCE）刺激对BALB／c裸鼠皮肤一氧化氮（NO）含量的影响，并观察银杏叶提取物（GbE）和维生素E（VE）的保护作用。方法将132只BALB／e裸鼠随机分成空白对照组、溶剂对照组（橄榄油）、TCE组（20％TCE、40％TCE、80％TCE和100％TCE）、GbE保护组（0．1％GbE、1％GbE和10％GbE）和VE保护组（5％VE、10％VE和20％VE），每组11只，其中5只用于急性刺激试验，6只用于累积性刺激试验，观察皮肤刺激反应，并检测背部皮肤组织中NO的含量。结果（1）各浓度TCE处理后裸鼠背部皮肤均产生不同程度的红斑和水肿，随着TCE浓度的升高以及染毒时间延长，皮肤的炎症反应程度逐渐加重。10％GbE组和20％VE组皮肤基本保持完好状态。（2）在急性刺激试验中，80％、100％TCE组NO含量分别为（69．89±59．605）和（77．273±9．290）μmol／mg蛋白，与空白对照组和溶剂对照组比较，差异有统计学意义（P〈0．05或P〈0．01）；各浓度GbE和VE预处理均能降低NO含量，与100％TCE组比较，差异均有统计学意义（P〈0．05或P〈0．01）。（3）在累积性刺激试验中，80％、100％TCE组NO含量分别为（60．362±9．817）和（68．027±9．354）μmol／mg蛋白，与空白对照和溶剂对照组相比，差异均有统计学意义（P〈0．05或P〈0．01）；1％GbE和10％GbE组以及10％VE和20％VE组NO含量明显降低，与100％TCE组比较，差异有统计学意义（P〈0．05或P〈0．01）。结论TCE对BALB／c裸鼠皮肤产生刺激作用，并诱导皮肤中NO含量升高；GbE和VE对TCE所引起的皮肤损伤有一定的保护作用。

Objective To study the changes of nitric oxide （NO） in the BALB/c hairless mice skin after trichloroethylene （TCE） irritation and the protection of ginkgo biloha extract（GbE） and vitamin E （VE）. Methods 132 BALB/c hairless mice were randomly divided into blank control group, solvent group （olive oil） , TCE groups（20%TCE, 40%TCE, 80%TCE and IO0%TCE）,GbE groups（0.1%GbE,1%GhE and 10% GbE） and VE groups （5%VE, 10% VE and 20% VE）,with 11 animals in each group, 5 for acute irritation test and 6 for the cumulative irritation test. The skin irritation was observed, and the levels of NO in the dorsal skin of BALB/C hairless mice were detected. The kit of NO was used to detect the levels of NO in the dorsal skin of BALB/c hairless mice. Results （1） The skin presented erythema and edema after TCE irritation both in acute irritation and cumulative irritation test and the skin inflammation showed time-dose effect relationship; the mice skin was protected in GhE or VE groups. （2） In the acute stimulation test, the levels of NO in 80%TCE group （69.895±9.605 μmol/mg pro） and 100%TCE group （77.273±9.290 μmol/mg pro） were significantly different compared with blank control group and solvent control group （P〈0.05 or P〈 0.01）. In the protection group, the NO level were reduced, with the statistically significant differences. （3） In acute irritation test, the levels of NO in 80%TCE group （60.362±9.817 μmol/mg pro） and 100%TCE group （68.027 ±9.354 μmol/mg pro） were significantly different compared with blank control group and solvent control group,（P〈0.05 or P〈0.01）; In the protection group, 1% GbE ,10% GbE ,10% VE and 20%VE could reduce the levels of NO, with statistically significant differences. Conclusion TCE can produce the irritation on the dorsal skin of BALB/c hairless mice and induce the significant increase of the NO levels. GbE and VE can protect the skin from TCE irritation damage.