黄芪对破裂型椎间盘突出重吸收动物模型的影响

An experimental study on the influence of radix astragali on the ressorption of ruptured disc herniation

目的:研究破裂型椎间盘突出重吸收过程中免疫反应的作用机制,以及黄芪干预重吸收过程的作用机制。方法:将28只雄性SD大鼠,随机分为正常组、模型组、黄芪注射液治疗组和胸腺肽治疗组等4组。动物10d后处死,取髓核组织,进行HE染色观察形态结构变化,流式细胞仪检测T、B细胞含量。结果:相对于模型组,治疗组大鼠髓核组织的形态结构发生明显变化,T、B细胞含量比例明显大于模型组。结论:突出髓核组织能够引发自身免疫反应,黄芪对破裂型椎间盘突出的重吸收有一定促进作用,可能是通过增强自身免疫而发挥作用的。

Objective ：To investigate the possible mechanism of immune response in the resorption of the ruptured intervertebral disc herniation, and the possible mechanism of radix astragali on the resorption of the ruptured disc herniation. Methods：Twenty-eight male SD （Sprague-dawley）rats were chosen. The rats were randomly divided into 4 groups：the control group, model group, the group treated with radix astragali injection and the group treated whit thymic peptide. The rats were killed and discs were harvested 10 days after treatment. Flow cytometry and HE staining were used for analysis of cells and tissue. Resuits：Compared with the control group, the proportion of activated T cells （CD4＋ and CD8＋ ） and B cells were significantly higher in the two drug-treatment groups. Conclusion： Herniated nucleus pulposus attracts activated T and B cells and triggered an immune response. Radix astragali could strengthen the autoimmune response.