淫羊藿总黄酮对去卵巢大鼠骨组织OPG、OPGLmRNA表达的影响

Effect of total flavone of epimedium on expression of bone OPG,OPGL mRNA in ovariectomized rats

目的:探讨淫羊藿总黄酮(total flavone of epimedium,TFE)治疗骨质疏松症的分子机制,为传统中药的现代化和二次开发提供实验依据。方法:将60只4月龄健康雌性SD大鼠按随机数字表法分为对照组(A组,20只,行假手术处理),去卵巢组(OVX组,20只,切除卵巢后不给予淫羊藿处理),淫羊藿组(TFE组,20只,切除卵巢后给予淫羊藿灌胃)。所有大鼠术前及术后4周以DEXA骨密度仪检测L4骨密度变化(若BMD下降>20%,则骨质疏松模型建立)。模型建立后TFE组大鼠给予淫羊藿总黄酮(浓度30mg/ml,10ml/kg,1次/d)灌胃4周。所有大鼠处死前再行DEXA骨密度检测,过量麻醉法处死后取其股骨下部,切片匀浆提取骨组织中RNA,应用逆转录-聚合酶链反应(RT-PCR)技术检测骨组织护骨素(osteoprotegrin,OPG)、护骨素配体(OPGL) mRNA的表达。结果:①TFE组大鼠切除卵巢4周后,腰椎BMD均值降至(0.084±0.020)g/cm2,降幅>20%证明骨质疏松模型建立。TFE灌胃4周后其腰椎BMD提高至(0.112±0.009)g/cm2,与给药前比较有明显改善,差异有统计学意义(P<0.05);②TFE组大鼠骨组织中OPG mR-NA的表达较OVX组相比,明显增强,且有统计学差异(P<0.05),但对OPGL mRNA表达促进作用不明显,组间无统计学差异(P>0.05)。结论:TFE是通过促进骨组织中OPG mRNA的表达来抑制破骨细胞的分化和成熟,从而达到治疗骨质疏松症的目的。

To investigate the molecular mechanismofTFE（totalflavoneofepimedium） in the treatment of osteoporosis, and then provide experimental evidence for modernization and further development of TFE as an traditional Chinese medicine. Methods：Sixty healthy female SD rats with aged 4 months were randomly divided into three groups （including control group in which rats received sham surgery, OVX group in which ovariectomized rats didn＇t give any medicine after the removal of ovaries and TFE group in which ovariectomized rats administrated TFE ）, 20 rats in each group. Compared bone mineral density（BMD） between before operation and at 4th week after operation in order to verify the establishment of osteoporotic model （criteria：BMD decreased more than 20% at 4th week after operation）. The tats in TEF group were administrated total flavone of epimedium （concentration 30 mg/ml, 10 ml/kg, qd） orally for 4 weeks. After this, killed rats to harvest the lower part of the femur and detected BMD again. Applying the reverse transcriptase-polymerase chain reaction technique （RT-PCR） to detect expression of OPG, OPGL mRNA in bone tissue. Results： ①）At 4th week after ovariectomy, the mean BMD of lumbar vertebra in TFE group fell to （0.084 Э 0.020） g/cm2. Administrated with TFE for 4 weeks,the BMD increased to （0.112 Э 0.009） g/cm2. There was significant improvement compare with the OVX group （P〈0.05）. ②Compared between OVX group and TFE group, The OPG mRNA expression of TFE group obviously enhanced. There was significant difference in statistics （P〈 0.05）. However,the promotion for OPGL mRNA expression were detected between OVX group and TFE group, there was no significant difference in statistics （P〉0.05）. Conclusion：This study showed that TFE could inhibit differentiation and maturation of osteoclast through enhancing OPG mRNA expression, accordingly, to treat osteoporosis.