犬小口径组织工程化血管移植

Implantation of canine tissue engineering small-diameter blood vessel

目的研究利用小口径组织工程化血管进行血管移植的可能性。方法来源于骨髓间充质干细胞分化的血管平滑肌样细胞和血管内皮样细胞分层种植于胶原包埋的PGA复合支架上;将复合体种植于动物皮下构建初级组织工程化血管,然后移植组织工程化血管于股动脉,行相关检查,了解移植后血管的特点及细胞来源。结果组织工程化血管移植物通畅,管腔无瘤样扩张,血管搏动佳,内膜光滑,无血栓形成。HE染色见血管壁和正常生理性血管壁的结构相似,分内膜、中膜和外膜三层;免疫荧光观察证实血管壁细胞来源于所种植的种子细胞。结论组织工程血管可以移植于动物体内,该复合体具有与天然血管相似的结构和功能。

Objective To study the possibility of small diameter TEBV implantation into artery by surgical operation. Methods The mixture of seed cells and scaffold were produced by seeding of vascular smooth muscle-like cells and vascular endothelium-like cells derived from bone marrow mesenchymal stem cells on PGA／collagen scaffold. The cell scaffold mixture was subcutaneously implanted into donor dogs for 8 weeks. The TEBV grafts were used to replace a segment of femoral artery. Results In TEBVs grafts group, the grafts were patent and covered with endothelium-like cells, and there was no thrombus formation in the vessels. Histological analysis of the tissue engineering blood vessel wall also showed a typical artery structure, including endothelium, media and adventitia. Two weeks after implantation, the labeled seed ceils by Brdu were found in the three layers of the vessel wall too. Conclusion The tissue engineering vessel may be used to replace natural arteries, the architecture and function of the mixture resembles those of the natural arteries.