大鼠骨髓间充质干细胞体外诱导分化为肝细胞

In vitro differentiation of bone marrow mesenchymal stem cells into hepatocytes from rat

目的研究HGF、aFGF及OSM诱导MSCs成为肝细胞的能力及其在分化中的作用。方法用HGF、aFGF、OSM诱导分化MSCs后,用化学发光法、RT-PCR法及谷氨酸脱氢酶法检测细胞AFP、ALB及尿素的表达情况。结果除对照组外其余各组细胞均有AFP表达(P<0.05);对照组及a+O组无ALB、尿素的表达,其余各组出现ALB及尿素的表达(P<0.05)。结论HGF联合aFGF、OSM及aFGF能够诱导MSCs分化为表达AFP、ALB、尿素的肝细胞;aFGF与OSM在没有HGF存在时不能诱导MSCs向较为成熟的肝细胞的分化。

Objective To study the potential function of factor（aFGF） and oncostatin M （OSM） to induce MSCs hepatocyte growth factor （HGF）, acid fibroblast growth differentiate into hepatocytes. Methods MSCs were treated with HGF, aFGF and OSM. The expression of AFP, ALB and urea was examined by chemo-luminescent technique, RT-PCR method and glutamic acid dehydrogenase technique. Results AFP expression emerged in test groups（P 〈 0. 05 ）. ALB and urea expression was found in other groups but not control group nor a ＋ O group （P 〈 0. 05）. Conclusion HGF, OSM and aFGF can induce MSCs differentiate into hepatocytes with the expression of AFP, ALB and excreting urea, while aFGF and OSM can not induce MSCs differentiate into mature hepatocyte without HGF.