组织工程化干细胞移植治疗大鼠急性脊髓损伤的研究

Experimental study on transplantation of tissue engineering stem cells for rat acute spinal cord injury models

目的:探讨自体骨髓间充质干细胞(BMSCs)移植对脊髓损伤(SCI)大鼠功能恢复的影响。方法:54只成年Wistar大鼠随机分成实验组(干细胞移植组)、DMEM组、空白对照组。用按Bregman法方法制作脊髓损伤模型。7d后干细胞移植组注入经体外传代培养诱导的骨髓间充质干细胞悬液,DMEM组在脊髓损伤处注入DMEM培养液,空白对照组只做损伤模型不做任何处理。处理后第1、4、8、12周分别对两组大鼠进行动物BBB评分、斜板试验、后肢运动功能检测和运动诱发电位(MEP)检测。动物处死后做组织学观察。结果:处理后1周时,两组动物脊髓神经功能均无明显恢复;第4、8、12周时移植组大鼠运动诱发电位潜伏期和波幅明显恢复,斜板试验角度和BBB评分及后肢运动功能检测评分均高于对照组大鼠(P<0.01);DMEM组与对照组间P>0.05。与前一时间点比较,运动诱发电位潜伏期和波幅均有明显恢复,(P<0.01);斜板试验角度和BBB评分以及后肢运动功能检测评分在第8周与第12周间无显著差异(P>0.05)。结论:BMSCs移植可以促进大鼠损伤脊髓功能的恢复。

Objective： To investigate curative effects of bone marrow mesenchymal stem cells（BMSCs） transplantation on rat models with acute spinal cord injury （ASCI）. Method：BMSCs were cultured in vitro. Animal SCI at T10 level model was executed by Bregman method. Fifty-four adult Wistar rats models were allocated randomly into 3 groups, stem cells transplantation group, DMEM group and blank control group （BC）.Seven days after SCI, BMSCs suspension cultured and induced in vitro and DMEM culture solution were injected into around the site of semisection of spinal cord of models respectively as transplantation group and DMEM group. No treatment was used in BC group after SCI model was made. Animal ethology was investigated. MEP was used to assess the therapeutic outcome at the 1st,4th,Sth,12th weeks respectively after transplantation. Result： There was no sign of functional recovery in each group one week after transplantation. An obvious statistically significant improvement in neurological function outcome were noted in rats with BMSCs transplantation groups 4 weeks after surgery, by means of BBB scale, slope test and hind limb motor functional detection, as well as MEP amplitude and latency time （P〈0.01）; There was no statistically significant difference between BC group and DMEM group （P〉0.05）. Compared with the former time point, an obvious statistically significant improvement could be seen in MEP amplitude and MEP latency time. However there was no statistically significant difference in BBB scores,the angles of slope test and hind limb motor functional detection between the 8th and 12th week （P〉0.05）. Conclusion： BMSCs cultured in vitro transplantation had better effects in treating ASCI model rats compared with that in control group.