红花对兔肠缺血再灌注损伤后肠壁ICAM-1表达的影响

Effects of Safflor Injection on ACAM-1 in Rabbits following Intestine Ischemia/Reperfusion Injury

目的:观察红花注射液对兔肠缺血再灌注损伤后肠组织细胞间黏附因子1表达影响。方法:制备兔肠缺血再灌注损伤模型。30只日本大耳兔,随机均分为三组:假手术组(S组),缺血再灌注组(IR组)和缺血再灌注+红花注射液组(SI组)。肠组织常规病理染色观察,并采用免疫组织化学法检测各组肠组织标本细胞间黏附因子1表达的改变。结果:细胞间黏附因子1蛋白在各组肠组织血管内皮细胞中均有表达,尤其在黏膜下层中表达显著。S组兔肠表达较弱,IR组肠血管上皮细胞上细胞间黏附因子1蛋白表达增强,与S组比较,差异有显著性(P<0.01)。SI组与IR组比较明显下降(P<0.01)。光镜观察比较SI组与IR组可见,多数肠黏膜上皮细胞变性、坏死程度明显减轻。结论:红花在兔肠缺血再灌注损伤中能有效减少炎性渗出、抑制微循环通透性的增加,阻断肠缺血再灌注损伤的病理生理过程。

Objective To determine the inhibitory effect of safflor injection（Sal Ⅰ） on the level of intercellular adhesion molecule- 1 （ICAM - 1） following intestine ischemia and reperfusion injury in rabbits. Methods Thirty rabbits were randomly divided into three groups： control group（group S）, ischemia/reperfusion group （group IR） and Safflor injection group（group SI）. The intestinal structure characteristics were observed by HE chemistry staining. The intestine samples were obtained to measure the expression of ICAM - 1 by immunohistochemistry. Results ICAM - 1 was found expressed on intestinal vascular endothelial cells, especially on intestinal submucosa. ICAM- 1 was expressed in group IR higher significantly than that in group S. The level of ICAM - 1 in group SI were lower remarkably than that in group IR（ P 〈 0.01） Conclusion Safflor injection can effectively decrease expression of ICAM - 1, reduce the aggregation and activation of PMN in intestinal tissue of ischemia - reperfusion rats, which may be the pathophysiological mechanism of underlying intestinal mucosa injury and enhanced intestinal permeability.