摘要
目的:观察卡维地洛对大鼠心室肌细胞ICa,L电流的抑制作用,探讨卡维地洛抗心律失常的离子通道机制。方法:采用胶原酶酶解法分离大鼠心室肌细胞;采用膜片钳全细胞技术记录大鼠心室肌细胞ICa,L电流,观察细胞外液中加入卡维地洛后,ICa,L电流的变化。结果:对照组及卡维地洛组ICa,L密度分别为(pA/pF):11.25±1.57,3.89±0.59(P<0.05);卡维地洛组ICa,L的I-V曲线向上方移位。结论:卡维地洛能够对抗心律失常的机理之一与它能够阻断ICa,L有关。
Objective ：To observe the inhibitory action of carvedilol on L- type calcium channel current （ICa,L） of ventricular myocytes in rats, and to explore the ionic channel mechanism of antiarrhythmic effect of carvedilol.Methods： The ICa,L of ventricle cardiomyocytes isolated by collagenase method was recorded by using patch clamp technique. The influence of carvedilol on ICa,L was analyzed. Results： The ICa,L current densities （pA/pF） were 11.25 ±1.57 in control group and 3.89 ± 0.59 in carvedilol group respectively （ P 〈 0.05 ）, and the current - voltage relationship （I- V） curve was shifted upward in carvedilol group. Conclusion：The antiarrhythmic effects of carvedilol may be related with the inhibition of ICa,L in ventricular myocytes.
出处
《西北国防医学杂志》
CAS
2009年第2期94-96,共3页
Medical Journal of National Defending Forces in Northwest China
关键词
卡维地洛
心律失常
钙通道电流
膜片钳技术
Carvedilol
Arrhythmias
Calcium channel current
Patch clamp technique
