肺癌组织中抗氧化酶及氧化代谢物变化的定量分析

The Quantitative Analysis of the Oxidants/Antioxidants in the Tissues of Lung Cancer Patients

背景与目的抗氧化酶与肿瘤的关系逐渐受到重视。通过对113例原发性肺癌患者手术切除肿瘤组织和肿瘤远端正常肺组织的抗氧化酶和氧化代谢物及SOD mRNA及其蛋白表达量的测定,观察肺癌组织氧化/抗氧化状态的特点,为补充或完善肺癌发生和发展机制提供一定的依据。方法应用比色法测定肺癌组织抗氧化酶活性和氧化代谢物[包括:总超氧化物歧化酶(TSOD)(包括MnSOD和CuZnSOD)、谷胱甘肽过氧化物酶(GPx)、过氧化氢(H2O2)、丙二醛(MDA)]的含量,采用半定量PCR和WesternBlot法测定患者组织中MnSOD和CuZnSOD的mRNA和蛋白表达量。结果①与肿瘤远端正常肺组织相比,肺癌组织中的TSOD、CuZnSOD、GPx活性增高,H2O2、MDA降低(P<0.05或P<0.01);②肺腺癌组织中的氧化代谢物H2O2、MDA高于肺鳞癌组织(P<0.05或P<0.01);③与肿瘤远端正常肺组织相比,肺癌组织中MnSOD mRNA和蛋白表达量降低,CuZnSOD mRNA和蛋白表达量增高(P<0.01)。结论肺癌组织存在着氧化/抗氧化失衡。SOD的异常表达水平均出现细胞异常生长,提示氧化/抗氧化失衡与肺癌的发生和发展存在一定的关系。

Background and objective The relationship between antioxidants and tumor was gradually paid attention. The changes of activities and the levels of expression mRNA and protein of oxidants and antioxidants, were evaluated in 113 lung tumor and 64 tumor-free lung tissues collected between the years 2006 and 2007 from 113 individuals with surgically resectable lung cancer in order to understand the mechanism of the onset and development of lung cancer. Methods The activities of TSOD, MnSOD, CuZnSOD, GPx, H2O2 and MDA were determined by ehromometry. MnSOD, CuZnSOD mRNA and protein expression levels were evaluated by Reverse transcription polymerase chain reaction and Western Blot analysis in tumor tissues, as well as corresponding control tissues. Results Comparing with the levels of these indexes in the tumor-free tissues, the activities of TSOD, CuZnSOD, GPx were higher, H2O2 and MDA were lower in the tumor tissues （P〈0.05 or P〈0.01）. Comparing with the group of the squalnous cell carcinoma, H2O2, MDA were higher in the tumor tissues of the group of adenoearcinoma （P〈0.05 or P〈0.01）. There was less MnSOD mRNA and protein in tumor than in tumor-free lung. The contents of CuZnSOD mRNA and protein in tumor were less than those in tumor-free lung tissue （P〈0.01）. Conclusion The imbalance between the oxidants and antioxidants exists in the tissues of patients with lung cancer. The imbalance may have some relationship with the onset and development of lung cancer.