甲状旁腺素基因多态与2型糖尿病患者骨密度的关系

Association of parathyroid hormone gene polymorphism with bone mineral density in the subjects with type 2 diabetes mellitus

目的探讨甲状旁腺素（PTH）基因多态与中国北方汉族人2型糖尿病患者骨密度的关系。方法运用聚合酶链反应限制性片段长度多态性（PCR-RFLP）技术检测了2型糖尿病（T2DM）组104例,健康对照（CON）组102例,206例中国北方汉族人PTH基因多态性;采用双能X线吸收法骨密度仪（DEXA）测量骨密度（BMD）。结果甲状旁腺素（BSTB1位点）基因型和等位基因分布频率在2型糖尿病组与对照组间差异无显著性（P〉0.05）;在对照组及2型糖尿病组,Bb/bb基因型者L2-4和Neck部位BMD显著低于BB型（P〈0.05）,Troch及Wards三角区BMD差异无显著性（P〉0.05）。结论2型糖尿病患者PTH基因多态性（BSTB1位点）可能是预测骨量减少、骨质疏松易感性的遗传标志。

Objective To evaluate the effect of parathyroid hormone gene polymorphism on bone mineral density （BMD） of type 2 diabetic patients of indigenous Han of north Chinese. Methods The 206 cases with 104 with type 2 diabetes mellitus （T2DM group）and 102 healthy controls （CON group）.Polymorphism of parathyroid hormone gene was determined by PCR-RFLP method. Bone mineral density（BMD） was measured by dual-energy X-ray absorptiometry（DEXA）. Results There was no significant difference in the distribution of genotypes and allele frequncies of PTH between the type 2 diabetic and control groups（P〉0.05）.Subjects with ＂BB＂ genotype had higher BMD at lumbar spine and femoral neck than those with ＂Bb/bb＂ genotype in control and type 2 diabetic group （P〈0.05）,respectively. Conclusion In type 2 diabetic subjects,BST B1 polymorphism of PTH gene would be a useful genetic marker for lower BMD and the susceptibility to osteoporosis.