NAC和牛磺酸对高氧诱导的离体兔晶状体混浊的抑制作用(英文)

N-acetylcysteine and Taurine inhibit hyperoxia-induced cataract in rabbit lens

目的:研究高氧环境下N-乙酰半胱氨酸(NAC)和牛磺酸(Tau)对离体兔晶状体的作用。方法:新鲜兔晶状体24只随机分为对照组、高氧组、20mmol/L NAC处理组和80mmol/L Tau处理组。除对照组正常培养外,其余各组培养在高氧环境(氧浓度>80%)下4h/d×7。每天观察其透明度,培养7d后检测各项生化指标。结果:培养7d后高氧组晶状体出现重度皮质混浊,正常组和80mmol/L牛磺酸处理组中度皮质混浊,20mmol/L NAC处理组仅轻度皮质混浊;高氧组与对照组相比谷胱甘肽含量及Na, K-ATPase活性分别下降了37.8%(P<0.05)和53.5%(P<0.05),两药物处理组均有不同程度升高,20mmol/L NAC处理组较80mmol/L Tau处理组升高更明显;过氧化氢酶、谷胱甘肽还原酶活性及水溶性蛋白含量的变化在各组间没有统计学意义。结论:NAC对高氧环境下兔晶状体皮质混浊的抑制作用优于Tau。

AIM： To investigate the efficacy of N-acetylcysteine （NAC） and taurine （ Tau ） in preventing hyperoxia- induced the lens opacification and the changes of biochemical parameters on organ cultured rabbit lenses.METHODS： Twenty-four lenses from adult rabbits were divided into the control group, the hyperoxia-exposed group, the hyperoxia-exposed group containing 20mmol/L of NAC, the hyperoxia-exposed group containing 80mmol/L of Tau, respectively. The treated groups incubated with hyperoxia （PO2〉80%） for 4 hours per day throughout a 7-day period. Lens transparency, histology and enzymatic activities measurements were determined after this incubation. RESULTS： Gross morphological examination of these lenses revealed some severe cortical opacification in the hyperoxia-exposed group, moderate cortical opacification in the control group and the Tau treated group. There was minimal cortical opacification in the NAC treated group. The glutathione （GSH） content and the activity of Na, K- ATPase were significantly decreased in the hyperoxia-exposed group than that of the control group, by 37.8% （P〈0.05） and 53.5% （P〈0.05）, respectively. However, they were increased in the two treated groups, especially in the NAC treated group. There were no significant differences in the water-soluble protein content and the catalase and GSH reductase activities in all group lenses. CONCLUSION： Hyperoxia can induce the cortical opacification in the lens. The role hyperoxia-induced cataract is of NAC in the prevention of superior to Tau.