转化生长因子β及其受体在翼状胬肉中基因表达的定量检测(英文)

Quantitative detection of the expression level of transform-ing growth factor-β and its receptors in pterygium with RT-PCR

目的:定量检测转化生长因子-β(Transforming Growth Fac-tor-β,TGF-β)及其受体在翼状胬肉和正常球结膜组织中的表达水平,探讨其在翼状胬肉病变发生发展过程中的作用。方法:随机选取翼状胬肉患者30例,手术切除翼状胬肉组织,并留取患眼角膜上缘上方的正常球结膜组织作为自身对照,实时荧光定量PCR技术分析翼状胬肉和正常球结膜组织中TGF-β1、TGF-β2及其I型和II型受体相对于内参照基因18S的相对mRNA含量。结果:TGF-β1在正常球结膜和翼状胬肉组织中平均表达水平分别为4.26×10-7±1.45×10-7和10.67×10-7±7.47×10-7,TGF-β2在正常球结膜和翼状胬肉组织中表达水平分别为1.08×10-10±0.68×10-10和8.23×10-11±6.63×10-11。TGF-βⅠ型受体在正常球结膜和胬肉组织中平均表达水平分别为0. 003015 ±0. 0036和0. 000379 ±0.000281, TGF-βⅡ型受体在正常球结膜和胬肉组织中表达水平分别为5.33×10-5±5.05×10-5和1.002×10-5±9.04×10-6。TGF-β1在胬肉组织中表达水平比正常结膜中表达水平升高倍数为2.9±2.8,差异有统计学意义(P<0.01)。TGF-β2在胬肉组织中表达水平比正常结膜中表达水平升高倍数为7.5±1.4,差异有统计学意义(P<0.01)。TGF-βⅠ型受体在胬肉组织中表达水平比正常球结膜中明显降低,差异有统计学意义(P<0.05)。TGF-βⅡ型受体在胬肉组织中表达水平比正常球结膜中表达水平明显降低,差异有统计学意义(P<0.01)。结论:TGF-β1和TGF-β2在胬肉组织中表达水平均高于正常球结膜组织。TGF-β1在正常球结膜和胬肉组织中表达水平均远高于相应组织中的TGF-β2表达,但是TGF-β2在胬肉中表达水平升高变化幅度高于TGF-β1。TGF-βⅠ型和Ⅱ型受体在胬肉组织中表达水平均低于正常球结膜组织。TGF-βⅠ型受体在正常球结膜和胬肉组织中表达水平均高于相应组织中的TGF-βⅡ型受体表达,提示胬肉组织中Ⅰ型、Ⅱ型受体表达的降低可能导致TGF-β分泌的增加,从而导致翼状胬肉病变的发生发展,提示转化生长因子-β及其受体在翼状胬肉病变发生发展中可能起着重要的作用。

AIM; To quantitatively investigate transforming growth factor-β（TGF-β） and its receptors in normal bulbar conjunctival tissues and pterygium tissues.METHODS： Thirty cases of pterygium patients were randomly selected to undergo surgical resection of pterygium lesion, and the normal margin of bulbar con- junctival tissues were collected as control. Gene expres- sion was detected quantitatively by the method of quantitative real-time PCR （QRT-PCR） analysis.RESULTS： The expression level of TGF-β1 and TGF-β2 was 4.26 × 10^-7±1.45 ×10^-7 and 1.08×10^-10±0.68×10^-10 in normal bulbar conjunctival tissues, while 10.67×10^-7±7.47×10^-7 and 8.23×10^-11±6. 63×10^-11 in pterygium tissues, The expression level of TGF-βR I and TGF-βR Ⅱ was 0. 003015±0. 0036 and 5.33 ×10^-5 ± 5.05×10^-5 in normal bulbar conjunctival tissues, while 0. 000379±0. 000281 and 1.002×10^5±9.04×10^-6 in pterygium tissues. The expression level of TGF-β1 and TGF-β2 in pterygium was elevated （P〈0.01）, TGF-β1 expression level in pterygium increase 2.9±2.8 times than in normal conjunctiva. TGF- β2 expression level in pterygium increase 7.5±1.4 times than in normal conjunctiva. The expression level of TGF-βR I in pterygium was significantly lower （ P〈 0.05）. The expression level of TGF-βRⅡ in pterygium was significantly lower （ P〈0.01）. CONCLUSION： QRT-PCR is an effective method to quantitatively detect gone expression in eye. The upregulation of TGF-β1 and TGF-β2 and downregulation of their receptors expression may play an important role in the pathogenesis of pterygium, which is noteworthy further investigation in diagnosis and treatment of pterygium.