好氧反硝化菌的筛选及其脱氮除磷性质的研究

Isolation of an Aerobic Denitrifying Bacterial Strain and Its Characterization in Nitrogen and Phosphorus Removal

利用富集培养基,从用生活污水驯化后的活性污泥中筛选得到一株具有好氧反硝化兼具除磷功能的细菌。通过形态学及生理生化指标鉴定其为假单胞菌属。利用此好氧反硝化菌处理模拟废水及生活废水,通过监测总氮、无机磷及CODcr变化确定在C/N摩尔比为3:1、接种量为10%、pH6.8、30°C条件下处理2d,该菌株脱氮、除磷及去除有机物的效果最佳,活性污泥经此好氧反硝化菌强化后,对生活废水的处理能力得到明显提升。

Utilizing the enrichment substrate, an aerobic denitfifying bacterial strain with the capability of phosphorus removal was screened from the activated sludge which had been domesticated by actual living sewage. By the morphological observing and identification of the physiological and biochemical indexes, the strain was identified to belong to Pseudomonas. The aerobic denitrifying bacterial strain was applied to deal with the simulated and actual living wastewater. By inspecting the changes of total nitrogen, inorganic phosphorus and CODcr in the wastewater, it was ascertained that the optimal wastewater treatment conditions of the bacterial strain were C/N= 3, inoculated ratio= 10%, pH 6.8, 30℃, treatment time= 2 d. After the activated sludge was strengthened by the aerobic denitrifying bacterial strain, its treatment capability for actual living sewage was improved, evidently.