不同声强超声波辐射对血管内皮细胞Ca^(2+)浓度的影响

Effects of Different Intensities of 800 kHz Ultrasound Radiation on Accumulation of Ca^(2+) in ECV-304 Cells

目的:研究800kHz不同声强的超声波辐射对人脐静脉血管内皮细胞(ECV-304)内钙离子浓度的影响,探讨超声波的作用机制。方法:将ECV-304细胞接种于细胞爬片上,培养后分为对照组、假辐射组和功率密度为0.20、0.40、0.60和0.80W/cm2占空比30%的脉冲超声波辐射组(超声波组)。超声波组各声强辐射5min后,与其他各组均采用钙离子荧光探针加载结合激光扫描共聚焦显微镜观察血管内皮细胞内钙离子浓度的变化。结果:假辐射组细胞内的钙离子浓度与对照组比较差异无显性意义,而0.20、0.40、0.60和0.80W/cm2的超声波组细胞内钙离子浓度均高于对照组(P<0.01)。超声波强度越高钙离子的变化越明显;各功率密度间比较,0.80W/cm2点明显高于0.20W/cm2点(P<0.05),其余点间两两比较无明显差异。结论:800kHz不同强度的超声波辐射,可导致ECV-304细胞内钙离子不同程度的超载,细胞内钙离子的变化与超声波辐射强度呈正相关。

Objective：To study the accumulation of Ca^2＋ in EVC-304 cells exposed to different intensities of 800 kHz ultrasound radiation and to explore the action mechanisms of ultrasound. Methods：After primary culture on coverglass, the EVC-304 cells were divided into 5 groups. Cells in experimental groups were exposed to 800 kHz ultrasound, 30% duty cycle, with power intensity output of 0. 20, 0.40, 0. 60 and 0. 80 W/cm^2 respectively for 5 min. The cells in false exposure group were managed in the same environment as in experimental groups with power intensity output of 0 W/cm^2. Fluo-3/AM fluorescence technique and laser scanning confocal microscope were used to measure the intracellular calcium levels in ECV-304 cells. Results：The accumulation of calcium ion in false exposure group showed a same level as in control group. However, intraeellular calcium ion levels inall experimental groups were higher than in control group （P〈0. 01）. The accumulation of calcium ion in 0.80 W/cm^2 group was obviously significantly in- creased as compared with 0. 20 W/cm^2 group. The higher power intensity output, the more Ca^2＋ release observed in the ECV-304 cells. Conclusion：800 kHz ultrasound radiation with different power intensities can increase the accumulation of intracellular Ca^2＋ in ECV-304 cells. The increase of intracellular Ca^2＋ is positively related to the intensity of ultrasound radiation.