HPLC-MS/MS法同时测定近岸底栖生物中15种磺胺类抗生素残留量

Simultaneous Determination of Fifteen Sulfonamide Residues in Macrobenthos by High-Performance Liquid Chromatography Tandem Mass Spectrometry

采用液-液萃取和固相萃取方法处理生物样品,建立以ESI(+)和SRM为基础的快速检测15种磺胺的HPLC-MS/MS方法,并采用该方法对大连周边海域底栖生物中磺胺的残留状况进行了分析.结果表明:近岸底栖生物中磺胺噻唑(STZ)、磺胺喹啉(SQX)、磺胺嘧啶(SDZ)和磺胺甲基异噁唑(SMZ)等检出率较高,质量分数为1.39～143.29 ng/g.说明在我国近岸底栖生物中已经有一定量的磺胺类抗生素的残留,并威胁到水生生物的安全,因此应加强对近岸海洋环境中抗生素残留的监测与管理.

This study used a rapid method for the determination of 15 kinds of sulfonamides （SAs） in macrobenthos using LLE-SPE, and LC-MS/MS with positive ESI （ESI（ ＋ ）） and selected reaction monitoring （SRM） mode. The method was applied to quantify the SAs residue in macrobenthos around Dalian. The results showed that sulfathiazole （STZ）, sulfathiazole （SQX）, sulfadiazine （SDZ） and sulfamethoxazole （SMZ） were frequently found in macrobenthos in a concentration range between 1.39 and 143.29 ng/g. This indicated that there was antibiotics residue in macrobenthos in the sea environment, which threatens the safety of the halobios. Therefore, strengthening the monitoring and managment of antibiotics residues in the sea environment is important.