反相高效液相色谱法测定家兔粪便中芒果苷的含量

Determination of Mangiferin in Dejecta of Rabbits by RP-HPLC

为研究家兔一次口服芒果苷后芒果苷在粪便中的排泄情况,应用反相高效液相色谱法测定芒果苷给予家兔口服后,第1～6d粪便中芒果苷的含量。家兔口服芒果苷后第1～2d可在粪便中测出原型芒果苷,第1d的排出率为20.3217%,第2d的排出率为4.1183%,第3d以后在粪便中不再检测出原型芒果苷,芒果苷粪便总排出率为24.44%;芒果苷含测方法的回收率为99.87%,RSD为1.92%。说明本实验建立的反相高效液相色谱法测定粪便中芒果苷浓度方法准确灵敏、重现性好。芒果苷经家兔一次性口服后,约有25%通过粪便以原型形式排泄。

To investigate the excretion process of mangiferin after single oral administration in rabbit, RP-HPLC was used to determinate the mangife prototype of mangifein was detected day. The fecal excretion rates were fin in dejecta of rabbits in 6 days after administration. It in the first day and the second day after administration, 20. 321%, 4. 1183% respectively, and the total fecal is resulted that the instead of the third excretion rate was 24.44%. The recovery rate of mangiferin was 99.87% ,RSD was 1.92%. It is conclused that a sensitive accurate and reproducible method for the concentration measurement of mangiferin in the dejecta of rabbit is developed.