丙型肝炎病毒抗体检测试剂的比对研究

Study on Comparison of Reagents for Hepatitis C Antibody Detection

〔目的〕通过对丙型肝炎病毒抗体检测试剂的比对检测,探讨诊断试剂盒的性能效果。〔方法〕采用6种第三代丙型肝炎病毒抗体酶联免疫吸附试验(ELISA)试剂检测抗-HCV,并应用逆转录聚合酶链反应(RT-PCR)检测HCV-RNA作为验证试验。〔结果〕对2005—2007年518份血清(包括抗-HCV检测阳性血清)进行检测,A试剂阳性检出率最高为5.79%,C、D试剂检出阳性率最低为3.47%。与12份PCR检测阳性结果最为吻合的试剂为A、F试剂,都是11份阳性(符合率为91.7℅)。〔结论〕A和F试剂是检测抗-HCV较理想的试剂。

Objective To study comparison of reagents for Hepatitis C antibody detection, and to explore the diagnostic reagent＇ effect. Method Six kinds of the third generation ELISA reagents were used to detect anti-HCV, and reverse transcription polymerase chain reaction （RT-PCR） was applied in testing HCV-RNA as a verification test. Result 518 samples of serum in 2005-2007 （including positive serum of anti-HCV detection） were tested. The reagent A had the highest rate, the positive rate was 5.79%. The reagent D and C had the lowest rate, the positive rate was 3.47%. And compared to 12 samples of PCR-positive results, the reagent A and F were highly matched, in which, 11 samples were PCR-positive （coincidence rate of 91.7%）. Conclusion A and Freagents are ideal reagent for anti-HCV detection.