二次免疫诱导C57BL/6J小鼠实验性自身免疫性脑脊髓炎动物模型的建立

Model Establishment of Experimental Allergic Encephalomyelitis and Its Pathological Characteristics in Secondary Immune C57BL/6J Mice

目的:探讨C57BL/6J小鼠建立实验性自身免疫性脑脊髓炎模型的可能性及其发病特点。方法:使用小鼠脑脊髓匀浆(spinal cord homogenate,SCH)免疫C57BL/6J小鼠,完全福(氏)免疫佐剂为免疫佐剂,并在尾静脉注射百日咳杆菌,建立EAE模型。结果:自制SCH免疫C57BL/6J小鼠可见明显脱髓鞘改变。两次免疫法与一次免疫法相比,发病率较高(P>0.05),神经功能评分较高(P>0.05),发病时间延长(P>0.05)。结论:SCH免疫C57BL/6J小鼠的EAE动物模型,主要表现为急性单相病程,从临床表现和病理学特点来看符合人类MS的病理特点,值得在以后的研究中进一步研究探讨。

Objective： To establish an experimental autoimmune encephalomyelitis （EAE） model in C57BL/6J mice, providing a basis for studying the immunopathological mechanism and experimental therapy of multiple sclerosis. Methods： Female C57BL/6J mice were immunized with spinal cord homogenate to establish EAE model. Results： Compared with one times immune model group, Spinal cord homogenate immunity C57BL/6J were higher Morbidity（ P 〉 0.05） and longer time of incidence（ P 〉 0.05）. Light microscopy showed there were abundant inflammatory cells infiltrated in the cerebral and spinal cord tissues in EAE mice, with evident demyelination in white matter. Conclusion： Spinal cord homogenate immunity C57BL/6J were showed acute single phase course, and which bears a resemblance to the features of multiple sclerosis, and might be a better model for the study on multiple sclerosis.