赤芍对家兔颈动脉损伤后血管NADPH氧化酶亚单位p22phox表达影响

Radix Paeoniae Rubra Inhibits NADPH Oxidase P22Phox Expression and Reduces Neointimal Hyperplasia after Balloon Injury in Cholesterol-Fed Rabbits

目的:观察赤芍对血管内膜损伤后还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)(NADPH Oxidase)亚单位p22phox mRNA表达的影响。方法:采用Clowes方法建立兔颈总动脉球囊损伤模型,并给予赤芍提取物。图像分析系统测定新生内膜面积,原位杂交及RT-PCR检测NADPH Oxidase P22Phox mRNA表达。结果:赤芍可显著减低NADPH Oxidase P22Phox mRNA表达,并减轻内膜增殖程度。结论:赤芍抑制高脂喂养兔颈动脉球囊损伤后NADPH Oxidase P22Phox mRNA表达及内膜增殖程度可能与赤芍的抗氧化活性有关。

Objective ：To observe the effect of Radix Paeoniae Rubra（RPR） on NADPH Oxidase P22Phox mRNA expression and neointimal hyperplasia after carotid artery balloon injury in cholesterol - fed rabbits. Methods ： The rabbit model of carotid balloon injury was established adopting clowes method, and treated with extract of RPR The area of new genesic intima was measured by image assay. NADPH Oxidase P22Phox expression was determined by in situ hybridization and RT- PCR. Results ：RPR significantly reduced NADPH Oxidase P22Phox mRNA expression in the carotid artery （ P 〈 0.05 or P 〈 0.01 ）. Conclusion ： RPR inhibits NADPH Oxidase P22Phox mRNA expression, and modestly reduces neointimal hyperplasia. The decreased intimal response might be partly attributed to its antioxidant effects.