山东丹参脂溶性组分HPLC指纹图谱及有效成分含量测定

The Fingerprint of Liposoluble Components and Determination Effective of Salvia miltiorrhiza Bge.in Shandong

目的:研究丹参脂溶性组分的HPLC指纹图谱,建立同时测定丹参中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ的HPLC分析方法。方法:采用HPLC法,以乙腈—水溶液为流动相进行梯度洗脱。结果:线性范围内3个组分的标准曲线呈良好的线性关系,平均加样回收率均在98%～103%。所有化合物的精密度和重复性的RSD均<3%,并建立了山东丹参脂溶性组分的HPLC标准指纹图谱。结论:该分析方法稳定可靠、重复性好,可同时分析丹参中的3个不同化合物,可为山东产丹参脂溶性组分指纹图谱及其质量控制的研究提供参考。

Objective：To study the HPLC fingerprint of liposoluble components of the Salvia miltiorrhiza Bge. , establish a method for the determination of tanshinone Ⅱ A, cryptotanshinone, tanshinone Ⅰ. Methods ： The mobile phase consisted of acetonitrile and water was used as gradient elution for separation with HPLC. Results： the three components of the standards was a good linear relationship with linear range, the average recovery are 98% - 103%. The RSD of precision and repeatability of the sample is less than 3% ,and set up the standard fingerprint of the liposoluble components of Salvia miltiorrhiza Bge. in Shandong. Conclusion：This analysis is stable and reproducible, can be used to analyze the three compounds of Salvia miltiorrhiza Bge. and make a basie of the fingerprint of the liposoluble components of Salvia miltiorrhiza Bge. in Shandong.