摘要
目的:探讨丹参酮ⅡA(TanⅡA)对局灶性脑缺血再灌注损伤大鼠脑组织单核细胞趋化蛋白-1(MCP-1)和肿瘤坏死因子-α(TNF-α)含量的影响。方法:将大鼠随机分为假手术组、缺血再灌注组、TanⅡA低剂量治疗组和TanⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。TanⅡA高、低剂量治疗组于术前连续灌胃给予高、低剂量TanⅡA3天,每天1次。各组于脑缺血90min再灌注24h进行2,3,5-三苯基氯化四氮唑(TTC)染色观察脑梗死体积,采用酶联免疫吸附试验(ELISA)法检测检测脑组织MCP-1和TNF-α含量的变化。结果:(1)TanⅡA治疗组脑梗死体积较缺血再灌注组减少,高、低剂量组之间差异亦具有显著性(P<0.05)。(2)与假手术组比较,缺血再灌注组脑组织MCP-1和TNF-α含量明显升高;与缺血再灌注组比较,TanⅡA高、低剂量治疗组脑组织MCP-1和NTNF-α含量均明显降低,高、低剂量组之间差异具有显著性(P<0.05)。结论:降低缺血再灌注损伤脑组织MCP-1和TNF-α含量,抑制再灌注损伤炎症反应,可能是TanⅡA发挥脑保护重要途径之一。
Objective ： To study effect of Tanshinone Ⅱ A （Tan Ⅱ A） on the contents of MCP - 1 and TNF - α following cerebral ischemia reperfusion（I/R） injury in rats. Methods：In this experiment rats were randomly divided into 4 groups, which were sham operated group,I/R group,low dose Tan Ⅱ A treated group and high dose Tan Ⅱ A treated group. The focal middle cerebral arterymocclusion（MCAO） model was made by suture -occluded method. Rats were pretreated with Tan Ⅱ A,ig for 3d,respectively before MCAO. After 90min MCAO following 24h of reperfusion,Trc staining was investigated. The contents of MCP- 1 and TNF- α,was also investigated with using ELISA. Results： （1）Compared with that of I/R group ,low and high dose Tan Ⅱ A treated group dose -dependently reduced cerebral infarction（ all P 〈 0. 05 ）. （2） Compared with sham operated group, the contents of MCP - 1 and TNF - α increased at 24h of reperfusion in the ischemic territory （ P 〈 0.05 ）. Compared with I/R group,low and high dose Tan Ⅱ A treated group reduced the contents of MCP - 1 and TNF-α dosedependently（ P 〈 0.05 ）. Conclusion ：Tan Ⅱ A may reduced cerebral ischemia- reperfusion injure by reducing the contents of MCP - 1 and TNF - α dose - dependently. It plays protective effect on cerebral ischemia injury.
出处
《中华中医药学刊》
CAS
2009年第1期160-162,共3页
Chinese Archives of Traditional Chinese Medicine
基金
广西自然科学基金资助项目(0542110)
