HPLC法研究配伍对肾通颗粒中淫羊藿苷溶出量的影响

Study on Influence of Compatibility on Icariin in Shentong Granules by HPLC

目的:建立HPLC法测定肾通颗粒中淫羊藿配伍知母、生地后各配伍煎液的淫羊藿苷含量的分析方法,研究药材不同配伍对淫羊藿苷溶出量的影响。方法:采用AlltechC18柱(4.6mm×250mm,5μm),乙腈-0.1%冰醋酸(30∶70)为流动相,紫外检测波长270nm,柱温30℃,流速1.0mL·min-1。结果:该法简便、准确、灵敏,其他成分对测定无干扰,可用于配伍研究中淫羊藿苷的含量测定。结论:淫羊藿与知母、生地配伍后淫羊藿苷的溶出量有显著性下降;三者不同配伍比例对淫羊藿苷溶出量无显著性影响。

Objective：To set up a quantitative method of icariin in decoctions prepared from various combinations of Herba Epimedii, Rhizoma Anemarrhenae and Radix Rehmanniae by HPLC in Shentong Granules and to determine the change of icariin contents in different decoctions and to study the influence of compatibility on the contents of icariin in decoctions. Methods：An Alltech C18 column（ 4. 6mm × 250mm ,5μm）was used with a mobile phase of acetonitrile -0. 1% acetic acid（ 30： 70） and a UV detector at the wavelength of 270nm. The column temperature was 30℃ and the flow rate was 1.0mL· min^-1. Results ：The method was simple, accurate, sensitive and was not interfered by other constituents in the prescription. It could be used to determine the contents of icariin in compatibility study. Conclusion： The amount of ieariin was significantly decreased when Herba Epimedii was decocted with Rhizoma Anemarrhenae and Radix Rehmanniae. The influence that Herba Epimedii was decocted with Rhizoma Anemarrhenae and Radix Rehmanniae at different amount levels was not significant.