电针督脉穴对局灶性脑缺血再灌注大鼠大脑基因表达谱的影响

Influence of Electroacupuncture-treated Du Meridian's Points on Cerebrd Ischemia/Reperfusion Pat's Cerebral Gene Etpression

目的:应用基因芯片技术研究电针对局灶性脑缺血再灌注大鼠脑组织基因表达谱的影响。在分子水平探讨电针治疗脑缺血再灌注损伤的作用机制。方法:大脑中动脉埋线法制备大鼠脑缺血再灌注模型,缺血2h再灌注24h后取受损脑组织提取总RNA。用大鼠全基因组寡核苷酸微阵列芯片检测电针"水沟"、"百会"穴对局灶性脑缺血再灌注大鼠大脑基因表达谱的影响。结果:局灶性脑缺血再灌注大鼠与假手术组大鼠脑组织基因表达谱相比,有175个差异表达基因,其中115个基因表达增高,60个基因表达下调;电针组与局灶性脑缺血再灌注大鼠脑组织基因表达谱相比,有74个差异表达基因,其中26个基因表达增高,48个基因表达下调。结论:本研究筛选出大量的差异表达基因,这些基因可能参与脑缺血再灌后脑损伤的发生发展过程,并参与了针灸抗损伤的机制。

Objective：To investigate the changes of the cerebral gene expression in ischemia and electroaeupuncture （EA）-treated ischemia. Methods：A focal cerebral ischemia/reperfusion rat model was performed with a modified middle cerebral artery occlusion method. Total RNA was extracted from the injured hemisphere at 24h after 2h transient middle cerebral artery occlusion（MCAo） and gene expression was analyzed by GeneChip. Results：Among the nearly 5700 genes, 175 genes were affected after ischemia/reperfusion injury,in which 115 genes were upregulated, and 60 genes were down- regulated. In EA-treated rats,74 genes were affected ,in which 26 genes were upregulated, and 48 genes were downregulated. Conclusion ：These results suggest that some genes may be involved in the pathologic mechanisms of cerebral ischemia/reperfusion and the therapy mechanisms of acupuncture anti-ischemie/reperfusion injury. Mieroarray： analysis may provide a framework for understanding these complicated mechanisms.