参麦注射液对急性脑缺血并发肺损伤大鼠血清TNF-α及IL-8的影响

Effects of the Shenmaiinjection on TNF-α and IL-8 in Rats With the Lung Injury Induced by Cerebral Ischemia-reperfusion

目的:观察参麦注射液对脑缺血再灌注后肺损伤大鼠血清TNF-α及IL-8水平的影响。方法:本实验采用40只wistar大鼠,雌雄各半,随机分为4组:正常组(空白组)、缺血再灌注组(模型组)、参麦+缺血再灌注组(参麦组)、丹参+缺血再灌注组(丹参组),观察脑缺血2h,再灌注4h后,各组动物肺组织病理、动脉血气分析及血清中TNF-α及IL-8含量的变化。结果:模型组肺组织出现明显的病理损伤,参麦组和丹参组肺损伤均明显改善。模型组PO2明显低于空白组,参麦组与丹参组PO2均显著高于模型组。模型组TNF-α及IL-8含量明显高于空白组,参麦组、丹参组明显低于模型组,参麦组与丹参组比较,血浆TNF-α及IL-8含量明显降低,有显著性差异。结论:脑缺血再灌注可导致大鼠急性肺损伤,参麦注射液和丹参注射液均能改善肺损伤,可能与抑制炎症过程中细胞因子的表达有关,并且以参麦注射液作用较佳。

Objective：To investigate the protective effects of Shenmaiin-jection on lung injury induced by cerebral ischemia-reperfusion in rats based on the changes in TNF - α and IL - 8. Methods ：The wister ratswere divided into model- group（MG） ,con-trolgroup（CG） ,Red Sage Root（R/G） ,Shen mai injection group（SIG） ,The pathological change in pulmonary tissue, the lung function including P（ O2 ）, p（ co2 ）, the contents of were endothelin inserum were observed in all the rats which were subject 2hours cerebral ischemia then reperfusion for 4 hours. Results ： Pulmonary injury was obvious in MG,but it was obviously improved in the SIG and RIG. The P（ O2 ）MG was lower obviously than that in CGand the SIC and RIG. The content of serum tumors necrosis factor（ TNF- α）and interleukin（ IL- 8）were lower in the SIG and the RIG than. in the MG. Conclusion：Pulmonary injury can be induced by cerebral ischemia reperfusion in rats, which can be improved by the use of Shen mai injection and Red Sage Root injection, and the Shenmai injection was better one. One of the protective effect may be related to regulate the expression of inflammatory factor responses after the actutc cerebral ischemia.