康莱特注射液抗恶性肿瘤肝转移疗效及机理的实验研究

A Research on the KangLaiTe Lnjection in Treating the Liver Metastasis

目的:通过接种W256癌肉瘤建立Wistar大鼠肝癌转移模型,观察康莱特注射液与肝动脉结扎法对大鼠荷瘤脾重与肝癌转移灶的抑制效果,并测定其对瘤组织内基质金属蛋白酶(MMP-1)及肝细胞生长因子(HGF)表达的影响,探讨中西医结合防治肝癌转移的新途径。方法:在Wistar大鼠脾下极注射W256癌肉瘤细胞悬液,建立肝癌转移模型。随机分为4组:对照组、动脉结扎组、康莱特组、康莱特+动脉结扎组。计算各组瘤重抑制率与肝癌转移抑制率,采用原位杂交技术检测肿瘤细胞内MMP-1和HGFmRNA表达水平。结果:康莱特和康莱特加动脉结扎组均显著抑制脾脏肿瘤生长并抑制肝脏瘤组织MMP-1mRNA表达,康莱特组能明显抑制肝癌转移灶产生,各治疗组大鼠瘤细胞HGFmRNA表达水平均低于对照组。结论:康莱特注射液可显著抑制原发灶生长与肝癌转移灶形成,抑制瘤细胞MMP-1表达是其抗肝转移的重要机制;康莱特注射液结合动脉结扎可使瘤细胞MMP-1表达减少,提示应优化两者结合途径。

Objective ：To observe the inhibiting effect of the KangLaiTe Injection（KLT） and hepatic arterial ligation on rat＇s spleen transplantation tumor and the Liver Metastasis, and to text the inhibiting effect of this two methods on the expressions of the matrix metalloproteinase（ MMP- 1 ）and the hepatocyte growth factor（HGF） in rats＇ metastatic hepatoma. Methods ： W256 cells were injected into lower part of Wistar rats＇ spleen to set up a liver metastasis model, all rats were randomly divided into four groups ： KLT group, KLT plus ligation group, ligation group and control group. Calculate the inhibition ratios of the spleen tumor＇s weight and the liver metastasis, use the in-situ hybridization method to observe the mRNA expressions of the MMP - 1 and the HGF in the tumor cell. Results ： Both the KLT group and the KLT plus ligation group can conspicuously inhibit the weight of spleen transplantation tumor and the mRNA expression of the MMP-1 in rats＇ metastatic hepatoma; The KLT group can also inhibit the formation rate of the liver metastasis; each group had a lower mRNA expression of the HGF contrast with the control group. Conclusion ： The KLT injection can inhibit both growth velocity of the spleen transplantation tumor and the liver Metastasis,lowing down the expression of the MMP - 1 could be an important suppressive mechanism of this drug. There is also a decreased expression of the MMP - 1 in the KLT plus ligation group which indicated that the two methods combination should be optimized to get a satisfactory curative effect.