紫七软肝片对复合因素致大鼠肝纤维化模型的动态影响

Ziqi Ruangan Pill's Influence on the Rat Hepatic Fibrosis Caused by Complicate Factors

目的:研究紫气软肝片对复合因素致大鼠肝纤维化模型的作用的动态影响。方法:采用"CCL4+高脂饮食+酒精"复合因素干预大鼠,分别观察第4周和8周大鼠肝纤维化相关指征的变化,同时观察紫气软肝片对不同时间点大鼠肝纤维化的作用机制,并与秋水仙碱对照。检测肝功能、羟脯氨酸(Hyp)、透明质酸(hyaluronicacid,HA)、层粘连蛋白(laminin,LN)、Ⅲ型前胶原氨端肽(procollagenⅢN-terminal peptide,PⅢP)、血栓素(TXB2)、前列环素(6-PGI)和血流变学指标等项目。结果:模型组大鼠从4周和8周末Hyp、HA、LN、PⅢP和TXB2、6-PGI及血流变学均有不同程度的改变,并呈现动态加重的过程,而紫气软肝片和秋水仙碱均有不同程度的治疗作用,尤以紫气软肝片作用明显(P<0.05或P<0.01)。结论:保肝、改善微循环,调节细胞外基质的合成与降解,抑制纤维组织增生是紫气软肝片有效防治肝纤维化的重要机制。

Objective ：The study concentrated on the Ziqi Ruanganpill＇s influence on the rat hepatic fibrosis caused by complicate factors. Methods：Rats model were treated with CCL4, food with high fat, alcohol..Rats hepatic fibrosis data were analyzed twice,the first time was after 4 weeks,the other 8 weeks. The mechanism of Ziqi Ruangan pill was estimated meanwhile,the control group was treated with colchicine. Liver function, Hyp, HA, LN, P Ⅲ P, TXB2,6- PGI and blood rheologyb value were estimated. Results ： Hyp, HA, LN, P Ⅲ P, TXB2 , 6 - PGI and blood rheology＇s value of rats in the model group had been progressing after 4 weeks and 8 weeks, while rats in the Ziqi Ruangan pill and colchicines group alleviated, especially those in Ziqi Ruangan pill group（ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion：The mechanism of Ziqi Ruangan pill therapy lies in the protection of liver function, mending blood circle, regulating the ECM, reducing fibrosis.