失血性休克-再灌注大鼠胃黏膜内皮素及一氧化氮含量的变化及丹参提取物F的作用

The Changes of ET and NO Contents in Gastric Mucosa Induced by Hemorrhagic-Shock Reperfusione and The Effects of DanShen Extract F.

目的：探讨丹参提取物F（Dan Shen Extract F,DSEF）在胃黏膜缺血-再灌注损伤中的保护作用与胃黏膜内皮素（Endothelin,ET）和一氧化氮（Nitric oxide,NO）的关系。方法：选用Wistar雄性大鼠随机分4组,假手术组（SC组,n=9）：进行放血前的所有处理步骤,静脉泵入生理盐水;单纯失血组（HE组,n=9）：放血后维持血压在2.67~4.00kPa,20min后处死,静脉泵入生理盐水;生理盐水组（NS组,n=10）和丹参提取物F组（DSEF组,n=12）均回输全部血液,维持20min后处死,NS组静脉泵入生理盐水,DSEF组泵入DSEF。结果：1）各组大鼠失血量及平均动脉压组间无显著差异（P〉0.05）,而休克前和再灌注后的平均动脉压组内也无显著差异（P〉0.05）;2）HE组大鼠未见胃黏膜有出血性损伤,DSEF组的损伤指数明显低于NS组（P〈0.01）;3）NS组NO含量明显低于SC组（P〈0.01）,DSEF组NO含量明显高于NS组（P〈0.01）。NS组ET含量明显增高,与SC组比有明显差异（P〈0.01）,DSEF组ET含量明显降低,与NS组比有明显差异（P〈0.01）。结论：1）胃黏膜NO含量下降,ET含量增加可能是其再灌注损伤的原因。2）DSEF通过维持胃黏膜NO与ET稳定,起到防止胃黏膜-再灌注损伤的作用。

Objective：to study the relation between the protective effects of DanShen Extract F（DSEF） on gastric mucosa injury due to Hemorrhagic - Shock reperfusion and the content of endothelin（ET） and nitric oxide（NO） in gastric mucosa. Methods：rats were randomly divided into four groups：group SC（ rats undergoing all procedures but hemorrhagic shock） ;group HE （ rats suffered from hemorrhagic shock for 20 min） , group NS and DSEF（ rats received blood reperfusion after shock for 20min and were killed after 20min）. Rats in group DSEF received intravenous infusion of DSEF,and norreal saline was infused（0. 03mL/min） in the other groups. Result： （1）blood lossing and MAP were similar among group HE, NS, DSEF（ P 〉 0. 05 ）, and there was no marked difference of MAP between before shock and after reperfusion （P 〉 0. 05）. （2） The index of injury in group DSEF was markedly lower than that in group NS （ P 〈 0. 01 ）, and the index in group HE was 0. （3）As compared with those in group SC, the level of NO decreased significantly, but the content of ET increased markedly in group NS（ P 〈 0. 01 ）. In comparsion with those in group NS,the level of NO rose obviously ,while the content of ET reduced dramatically in group DSEF（ P 〈 0. 01 ）. Conclusion： the gastric mucosal lesion caused by the reperfusion may be in close contact with the decreasing of NO and the increasing of ET in gastric mucosa and DSEF can inhibit the changes to protect gastric mucosa from reperfusion impairment.