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炮制对黄精薯蓣皂苷元影响的研究 被引量:19

Influence of Process on Diosgenin in Rhizoma Polygonati
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摘要 目的:研究炮制对黄精薯蓣皂苷元的影响。方法:采用薄层色谱法鉴定薯蓣皂苷元,并以高效液相法测定其含量。结果:薄层色谱显示炮制前后样品均在薯蓣皂苷元对照品相应位置出现斑点,且斑点清晰、重现性好;薯蓣皂苷元对照品线性回归方程y=11.666x-0.2834,在0.125~2.5μg范围内呈良好的线性关系(r=0.9992),平均回收率为100.04%,RSD值1.73%。结论:黄精炮制前后均含有薯蓣皂苷元,但炮制后黄精中薯蓣皂苷元含量下降,薯蓣皂苷元含量变化与黄精炮制前后药效变化的关联性有待于进一步研究。 Objective:To study the influence on diosgenin in Rhizoma polygonati of processing. Methods:TLC and HPLC were apply to identify the diosgenin and determine the content of diosgenin in Rhizoma polygonati and the proeessed products respectively. Results:The spots of diosgenin in Rhizoma polygonati and the processed products showed dearly. The regression equation was y = 11. 666x - 0. 2834 and The linear range was 0. 125 - 2. 5 g,g , r = 0. 9995. The average mean of the moethod was100. 04% ,RSD was 1.73%. Conclusion:The content of diosgenin in Rhizoma polygonnail has decreased after being processed. The relationship between the changes of diosgenin's contents and drug action remained further research.
出处 《中华中医药学刊》 CAS 2009年第3期538-540,共3页 Chinese Archives of Traditional Chinese Medicine
基金 江西省教育厅资助项目(GJJ08328) 江西省卫生厅资助项目(2006A58)
关键词 黄精 炮制 薯蓣皂苷元 影响 rhizoma polygonati processing diosgenin influence
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