一氧化氮合酶组化染色对复合皮神经再生的观察

The investigation on nerve regeneration of composite skin with NADPHdiaphorase histochemistry

目的：为探讨复合皮（ＣＳ）神经再生情况。方法：用ＮＡＤＰＨ黄递酶组织化学染色方法（ＮＤＰ）观察３７头猪１２块经酶处理的冷冻异体乳猪真皮＋自体表皮一次性重组ＣＳ和７块自体表皮（Ｃ）移植后不同时间点活检标本内一氧化氮合酶（ＮＯＳ）的分布。结果：ＣＳ移植术后８～１２ｗ神经纤维（Ｎｆ）开始再生；２０～２４ｗ，Ｎｆ超正常密度再生，乳头内似有Ｍｅｒｋｅｌ细胞和Ｍｅｉｓｎｅｒ小体结构形成；３２ｗ以后，Ｎｆ结构和功能重塑，轴突增粗，密度降低，６８ｗ时已接近正常皮肤Ｎｆ密度和排布。Ｃ组：神经再生受阻于皮下大量致密的胶原纤维，再生的Ｎｆ纤细分布不规律，呈间断不连续状，至６８ｗ时，仍无乳头和感觉小体结构形成。结论：①ＮＯＳ组化染色方法简单可靠，可清楚地反映皮肤Ｎｆ再生经过；②ＣＳ移植后，神经再生和感觉重建迅速，Ｎｆ排布接近正常皮肤，与异体乳猪真皮引导性组织再生作用密切相关；③ＮＯ参与了移植皮片内神经再生及其再生后的感觉形成、整合和传递。

bjective:To study the nerve regeneration of composite skin(CS).Methods:We investigated the NOS positive site in the biopsy specimens of autoepidermis and CS which composed of autoepidermis and frozen new born pig allodermis.Results:The experiment showed that the nevous fibres regenerate in the eighth week after transplantation of CS,High density of new nevous fibres were observed as Merkel cells and Meissner body in the twentyfourth week.In the 32nd week,the structure and function of nevous fibres recovered,and axon were normalise,and the nerve fibre density became lower in the autoepidermis transplanting group.Nerve regeneration showed a amount of glialfibres,which were small and thin,and fibre threads were not continuous.In 68 weeks,there were no marked cells and Meissner bodies formed.Conclusions:The NOS histochemistry method is simple and reliable in reviewing Nf reproducing of skin.After CS transplantation,Nf regenerates quickly and NS recovers approximately to that of normal skin.NO may take part in Nf reproduction and sense integrating and delivery.