肺癌患者肺泡巨噬细胞一氧化氮活性研究

A study on the activity of nitric oxide in alveolarmacrophages from patients with lung cancer

用镀铜镉还原－Ｇｒｉｅｓｓ法检测肺癌患者ＢＡＬＦ及肺泡巨噬细胞（ＡＭ）培养上清液中ＮＯ水平；ＲＴ－ＰＣＲ检测ＡＭｉＮＯＳｍＲＮＡ表达。结果显示，肺癌组ＢＡＬＦ及ＡＭ培养上清液中ＮＯ水平均明显低于对照组。两组ＡＭｉＮＯＳｍＲＮＡ表达阳性率分别为６９％和９１％（Ｐ＞０．０５），但表达强度肺癌组明显弱于对照组（Ｐ＜０．０１）。经ＧＭ－ＣＳＦ刺激后，ＡＭｉＮＯＳ表达强度及培养上清液中ＮＯ水平均显著提高。提示肺癌局部ＡＭ抗肿瘤功能可能存在某些缺陷；

Nitrite and nitrate (NO_2^-/NO_2^-) in the bronchus alveolar lavage fluid (BALF) and the supernatants of incubated alveolar macrophages(AMs) from patients with primary lung cancer were measured by coppercoated cadmium reduction and Griess metehod. mRNA expression of AM induced nitric oxide synthase(iNOS) were analyzed by RTPCR. There was NO-2/NO-2 in BALF either from lung cancer patients or from control subjects. When compared with control group and the nontumorbearing lung, the level of NO-2/NO-2 was lower in BALF from the tumorbearing lung [5.18±1.1 vs 2.47±0.67nmol·mg protein-1 (P<0.01); 4.65±2.46 vs 2.47±0.67nmol·mg protein-1 (P<0.01)]. We also found a lower level of NO-2/NO-2 in the supernatants of incubated AMs from the lung of cancer patients than from control and nontumorbearing lung [95.03±21.76 vs 63.37±17.58nmol (P<0.01); 85.61±16.70 vs 63.37±17.58nmol (P<0.05)]. No significant difference existed between the mRNA expression of AM iNOS in lung cancer patients (69%) and that of control subjects (91%). After the AMs were stimulated with granulocytemacrophage colony stimulating factor (GMCSF), the level of NO-2/NO-2 in the supernatants was significantly increased (P<0.01); while the mRNA expression of AM iNOS from patients with lung cancer resulted in an increase of 16.85±7.58% vs 33.38±8.21% of control group (P<0.05). These observation suggest that some defects of antitumor function occur in the AMs at the tumor region. GMCSF can stimulate AMs and thus potentiate their NO activity.