清开灵注射液生物热活性指纹图谱的初步研究

Investigation on the Bioactivity Fingerprint of Qingkailing Injection by Microcalorimetry

目的研究清开灵注射液作用于金黄色葡萄球菌的生物热活性指纹图谱,探索清开灵注射液质量的生物活性评价新方法。方法采用微量量热法,在合适测试浓度下,分别测定各清开灵注射液样品生物热活性指纹图谱,并进行定性、定量分析。结果不同厂家及同厂家不同批次清开灵注射液样品的生物热活性指纹图谱相似度及热动力学参数存在不同程度差异,一个过期样品亦可被区分。结论该方法简便可靠,可为清开灵注射液的质量控制提供参考,为从活性角度研究中药注射液的质量一致性和稳定性提供了一种很好的思路。

OBJECTIVE To investigate the bioactivity fingerprint of Qingkailing Injection on staphylococcus aureus, and to search new method for evaluating bioactivity of Qingkailing Injection. METHODS The bioactivity fingerprints of different Qingkailing Injection on staphylococcus aureus were determined by microcalorimetry, analyzed qualitatively and quantitively. RESULTS The similarity and thermomechanical parameters were different between different factories and different batches. An expired sample was distinguished. CONCLUSION The result showed that this method is single, practicable, and reliable as a reference method for the quality control of Qingkailing Injection. A new direction was provided for investigating consistency and stability of traditional Chinese medicine injection.