衍生化GC-MS同时测定鱼腥草药材中9种脂肪酸的含量

Simultaneous Determination of Nine Fatty Acids in Houttynia cordata Thunb. by Derivatized GC-MS

目的建立衍生化GC-MS同时测定不同来源鱼腥草药材中9种脂肪酸甲酯化衍生物的含量。方法采用Inowax(0.32mm×30m,0.25μm)弹性石英毛细管色谱柱,程序升温,初温:110℃保持3min,以2℃·min-1升温至164℃,保持10min,再以8℃·min-1升温至210℃,保持10min。进样口温度为230℃,分流比为20∶1,进样量1μL,柱流速2.5mL·min-1,对不同来源的鱼腥草药材石油醚提取物的甲酯化样品采用SIM方式进行分析。结果月桂酸、肉豆蔻酸、棕榈酸、棕榈油酸、硬脂酸、油酸、α-亚麻酸、亚油酸、γ-亚油酸等9种脂肪酸均获得良好线性,并测定其在不同来源的鱼腥草药材中含量。结论方法准确、专属,能有效测定鱼腥草药材中含有的脂肪酸。

OBJECTIVE To develop a derivatized GC-MS method for the simultaneous determinations of methyl ester derivant of lauric acid, tetradecanoic acid, palmic acid, palmoleic acid, octadecanoic acid, oleic acid, α - linoleic acid, linoleate acid, γ-linoleate acid in Houttynia cordata Thunb. METHODS The analysis was carried on an AB-Inoawax capillary column （30 m × 0.32 mm, film thickness 0.25 μm）. The GC oven temperature was programmed with an initial temperature of 110 ℃, isothermal for 3 min, then increasing to 164℃ by 2℃.min^-1, holding it for 10 min, and increasing to 210 ℃ by 8 ℃min^-1, holding it for 10 min. The temperature of the injection port and interface was set at 230℃. Helium was used as the carrier gas, and flow rate was 2.5 mL.min^-1. One microlitre of the samples was injected in the 20 ： 1. RESULTS The good linearities of the nine fatty acids were obtained and the contents of them were determined. CONCLUSION This method was found to be selective, accurate and suitable for the determination of fatty acids in Houttynia cordata Thunb.