特发性血小板减少性紫癜患者IL-2受体的变化

Detection of interleukin2 receptor in patients withidiopathic thrombocytopenic purpura

应用ＥＬＩＳＡ法检测ＩＴＰ患者血清可溶性白细胞介素－２受体（ＳＩＬ－２Ｒ），用间接免疫荧光法检测ＰＨＡ活化的淋巴细胞白细胞介素－２受体（ｍＩＬ－２Ｒ）。结果表明，ＩＴＰ患者糖皮质激素治疗前ＳＩＬ－２Ｒ水平升高，治疗后降低；ＰＨＡ活化的ｍＩＬ－２Ｒ数较对照组降低（Ｐ＜０．０１），且与血清ＳＩＬ－２Ｒ水平无相关性。提示ＳＩＬ－２Ｒ水平升高可能是ＩＴＰ细胞免疫功能下降的原因之一。检测血清ＳＩＬ－２Ｒ水平可作为衡量体内细胞免疫活性的一个指标。

Using ELISA and indirect fluorescent antibody techniques(IFAT), the serum soluble interleukin2 receptor(sIL-2R) and membrane IL-2R(mIL2R) on lymphocyte cells induced by phytohemagglutinin (PHA) were studied in patients with idiopathic thrombocytopenic purpura(ITP). The results showed that the level of sIL-2R increased significantly before treatment and decreased after treatment; the percentages of mIL-2R induced by PHA decreased. There was no correlation between the level of serum sIL-2R and that of mIL-2R. Our observations suggest that increased level of serum sIL-2R in ITP patients may be one of causes evoking cellular immunological abnormalities.