阵发性睡眠性血红蛋白尿患者的淋巴细胞对红系和粒-巨噬系祖细胞的影响

Effects of Lymphocytes from Patients with Paroxysmal Nocturnal Hemoglobinuria (PNH) on Erythroid and Myeloid Progenitors

为了研究阵发性睡眠性血红蛋白尿（ＰＮＨ）患者的淋巴细胞对红系和粒－巨噬系祖细胞的影响，将ＰＮＨ患者的植物血凝素－淋巴细胞培养液（ＰＨＡ－ＬＣＭ）加入造血祖细胞培养基中，用于红系爆式集落形成单位（ＢＦＵ－Ｅ）和粒－巨噬系集落形成单位（ＣＦＵ－ＧＭ）的培养。结果在含有ＰＮＨ患者的ＰＨＡ－ＬＣＭ的培养条件下，ＰＮＨ患者骨髓ＢＦＵ－Ｅ和ＣＦＵ－ＧＭ集落数明显低于含有正常人ＰＨＡ－ＬＣＭ的培养条件下的集落数；正常人骨髓ＢＦＵ－Ｅ和ＣＦＵ－ＧＭ集落数亦明显低于含有正常人的ＰＨＡ－ＬＣＭ培养条件下的集落数。结果表明ＰＮＨ患者淋巴细胞对造血的支持作用减弱。

The purpose of this study was to investigate the effects of lymphocytes from patients with paroxysmal nocturnal hemoglobinuria (PNH) on erythroid and myeloid progenitors. Phytohaemaglutininstimulating lymphocytes conditioned medium (PNALCM) from PNH blood was added to the culture medium for the culture of PNH and normal burstforming unitserythroid (BFUE) and colonyforming unitsgranulocyte/macrophage(CFUGM). The results showed that the number of PNH bone marrow BFUE and CFUGM in the medium containing PHALCM from PNH blood was smaller than that form normal blood; that the number of normal bone marrow BFUE and CFUGM grown in the medium containing PHALCM from PNH blood decreased more than that from normal blood. It was suggested that diminished number of PNH bone marrow BFUE and CFUGM resulted in declined supporting effects of lymphocytes on hematopoiesis.