摘要
用基因工程重组的丙肝抗原包被于硝酸纤维素膜，建立了检测丙肝ＩｇＧ抗体的斑点免疫渗滤法。与ＥＬＩＳＡ试剂盒进行双盲式同步测定，二法检验结果差异无显著性。渗滤法简便快速，适用于各级医院，有很强的推广价值。
A dot immunofiltration assay (DIFA) of detecting HCV IgG antibody was established by coating recombined HCV antigen on the nitrocellulose membrane. The DIFA kit has passed through a test by HCV antibody sera panell of the National Center of Clinical Laboratroy Science. Parallel determination to ELISA kit showed an excellent coincidence. DIFA is simple and rapid, applicable in medical laboratories of different levels. Its extension for practical use is very valuable.
基金
湖北省重点资助
