摘要
为建立特异、敏感、快速而简单的结核病免疫诊断技术，制备了８株抗结核分枝杆菌的ＩｇＧ单克隆抗体，经ＥＬＩＳＡ法与结核分枝杆菌及８株非结核分枝杆菌的ＰＰＤ鉴定，筛选了其特异性及敏感性。与结核分枝杆菌聚合ＯＴ测定了其培养上清抗体滴度在１０－２以上。腹水抗体滴度在２×１０－４以上，用免疫转印技术确定了与其特异性结合的抗原相对分子质量分别为４２．７，２７．８ＫＵ。８株杂交瘤细胞的染色体计数为９８～１３０条不等。
We produced 8 IgG class specific monoclonal antibodies(McAb)to Mycobacterium tuberculosis for immunodiagnostic purposes. Specificity and sensitivity of 8 McAbs to various PPD of Mycobacteria were developed in ELISA systems. The chromosome numbers of hybridomas were 98￣130. Four of the McAbs (TB 20 ,TB 38 ,TB 41 and TB 109 ) were analysed by Western Blot. They showed a specificity restricted to the tuberculosis complex and could be divided into two groups according to their reactivity pattern with two different molecular size: TB 41 , TB 109 reacted with a 27.8 KU molecule and TB 20 , TB 38 reacted with a 42.7 KU molecule.
