大鼠颌下腺Mallory染色和免疫组织化学观察

Mallory stain and immunohistochemical observations on submandibular glands in rats

目的对大鼠颌下腺颗粒曲管（ｇｒａｎｕｌａｒｃｏｎｖｏｌｕｔｅｄｔｕｂｕｌｅ，ＧＣＴ）进行观察。方法本文用Ｍａｌｏｒｙ染色和免疫组织化学ＡＢＣ法。结果发现ＧＣＴ细胞的形态结构及细胞内分泌颗粒的数量有一定差异。此外ＧＣＴ细胞呈生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＯＭ）免疫反应阳性。结论表明大鼠颌下腺ＧＣＴ细胞内含有ＳＯＭ，对颌下腺复杂的分泌活动可能具有一定的调节作用。

Objective To distinguish the classifications of granular convoluted tubule (GCT) cells. Methods Specimens of submandibular glands in rats were dyeled and observed by Mallory stain and immunohistochemical ABC techniques. Results GCT cells could be divided into three types of large, small and narrow cells. Somatostatin (SOM) immunoreaction was positive among them. Conclusion SOM might play a regulatory role in complicated secretion of submandibular glands.